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頁籤選單縮合
| 題 名 | 山羊關節炎腦炎病毒於公羊精液之監測=Detection of Caprine Arthritis-encephalitis virus (CAEV) in Semen of Bucks |
|---|---|
| 作 者 | 章嘉潔; 吳昇陽; 吳昇陽; 章嘉潔; | 書刊名 | 畜產研究 |
| 卷 期 | 49:2 2016.06[民105.06] |
| 頁 次 | 頁121-129 |
| 分類號 | 437.58 |
| 關鍵詞 | 山羊; 山羊關節炎腦炎; 巢式聚合酶連鎖反應; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 人工授精為家畜生產性能改良之必要技術;雖然該技術已降低病原傳播風險,仍有研究持續進行確認風險評估, 本試驗旨在確認種公羊精液感染山羊關節炎腦炎病毒 (caprine arthritis encephalitis virus, CAEV) 狀況及巢式聚合?連 鎖反應 (nested polymerase chain reaction, nested PCR) 監測技術之建立,以提供控制本病方法之參考。以臺東某羊場 所飼養努比亞種羊 13 頭進行檢測,血清樣品採 CAEV/MVV Antibody Test Kit (CHEKIT) 商業套組,酵素結合免疫吸 附試驗原理 (enzyme linked immunosorbent assay, ELISA) 進 CAEV 抗體檢測,另將採集之血液及精液抽取 DNA 進行 nested PCR 檢測。精液樣品經離心分三部分:精漿 (seminal plasma, SP)、非精細胞 (non spermatic cells, NSC) 及精細 胞 (spermatozoa, SPZ),血液與精液樣品利用 nested PCR 檢測 CAEV 之 proviral DNA 片段,結果血清 ELISA CAEV 抗體檢測 10 頭陽性反應,血液樣品 nested PCR CAEV 之 proviral DNA 同樣有一致性檢測出,6 件精液樣品 CAEV proviral DNA 於 SP 及 NSC 分別被檢測出,另於 SPZ 部份 CAEV proviral DNA 均未檢測出,目前檢測 CAEV proviral DNA 存在精液非細胞部分及精漿,這研究證實 CAEV 存在於感染公羊的精液。 |
| 英文摘要 | Artificial insemination (AI) is essential to improve animal production performance. Although it greatly reduces the risk of pathogen transmission, some studies have been performed to quantify this risk. The aims of this experiment were to perform caprine arthritis encephalitis virus (CAEV) monitoring in semen, and to use a screening method named nested polymerase chain reaction (nested PCR) as a reference for the control of CAE. Blood samples of thirteen Nubian goats from a Taitung goat farm were used for test. The collected serum were evaluated for anti CAEV antibodies using a commercially available ELISA kit (Chekit ® CAEV/MVV, Liebefeld Bern, Switzerland). The DNAs were extracted from the blood and semen, and used nested PCR for viral detection. Semen samples were collected and separated into three distinct fractions: seminal fluid (SF), enriched non spermatozoa cell (NSC) and spermatozoa (SPZ). Nested PCR was used to detect the presence of CAEV proviral DNA in the blood and semen. Ten CAEV seropositive bucks were positive for nested PCR CAEV proviral DNA but only six semen samples were positive for CAEV DNA in non-spermatic cells and seminal plasma. No CAEV proviral DNA was identified in the spermatozoa fraction. The presence of CAEV proviral DNA in non spermatic cells and seminal plasma. This study clearly demonstrates the presence of proviral DNA in naturally infected caprine semen. |
本系統中英文摘要資訊取自各篇刊載內容。