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題名 | 人工感染山羊關節炎腦炎病毒後之免疫反應、抗體持續現象及病理學變化=The Persistent Immune Response and Pathological Changes of Goats Artificially Infected with Caprine Arthritis Encephalitis Virus |
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作者 | 韓佳洲; 李維誠; 林正忠; 簡茂盛; 劉正義; Han, Chia-chou; Lee, Wei-cheng; Lin, Cheng-chung; Chien, Maw-sheng; Liu, Cheng-i; |
期刊 | 臺灣畜牧獸醫學會會報 |
出版日期 | 19971200 |
卷期 | 67:3/4 民86.12 |
頁次 | 頁179-186 |
分類號 | 437.2 |
語文 | chi |
關鍵詞 | 山羊關節炎腦炎病毒; 免疫反應; 抗體持續現象; 病理學變化; Caprine arthritis encephalitis; Immune response; Pathology; |
中文摘要 | 山羊關節炎腦炎(caprine arthritis encepha1itis;CAE)病毒之持續感染現象及 抗體產生情形,為引致關節病變之主要機制所在。本實驗以 4 隻 CAE 陰性仔公羊 (Gl-G4) ,於皮下接種 CAEV-TC1 株病毒 (5x10 �� TCID50) 以探討其免疫反應及病理變化。對照組 包括自然感染陽性羊一頭 (G5) 及 CAE 陰性羊一頭 (G6)。實驗羊隻定期採取血液,利用西 方墨點法偵測抗體之出現及持續情形。 感染羊血清以確認 4 個主要病毒結構蛋白為判定標 準,包括病毒 gag 蛋白 p28-pl6-pl4、及 env 醣蛋白 gpl35。 Gl-G4 分別於感染後第 17 、33、29、39 天首先出現抗病毒 p28 蛋白之 lgG 抗體,並穩定而持續地出現於實驗全程; 繼 p28 後,按著出現的是抗 pl6、pl4 之 lgG 抗體。 陽性對照組羊隻 G5 抗 p28、pl6、 pl4 之抗體,自實驗開始即出現,並持續於全程,而抗 gp135 之 IgG 抗體並不明顯且不穩 定出現。 G3 及 G4 之抗 gp135 抗體則分別於感染後第 30 及 10 週出現。 實驗感染羊隻 Gl 及 G2 於感染後, 第 17 及 33 天解剖,除腕關節輕微出血外,其他臟器並無明顯病變 。G3 及 G4 在人工接種後 5 個月,腕關節開始有腫大現象,且隨感染時間之增長,病變愈 發明顯。 G3、G5 及 G4 羊隻於實驗後 1 及 2 年,犧牲解剖發現雙側腕關節面潰瘍,滑膜 結締組織增厚、肺臟尖葉、心葉及部分膈葉成不等度之堅實肉樣變化。組織病理學檢查則以 單核細胞浸潤、聚集之慢性進行性關節炎和間質性肺炎為主要病症。 |
英文摘要 | The understanding of the lentiviral persistence and immune responses is very important in the pathogenesis of carpal joint tension in caprine arthritis encephalitis (CAE) virus infection. Four months old CAE negative goat kids (G1-G4) were inoculated with CAEV-TC1 virus, subcutaneously, 5x10 �� TCID �t to investigate the immune responses and the persistence of antibodies. One negative control goat (G6) was kept living together with a naturally CAE virus infected goat (G5) in the same pen for horizontal transmission observation. The antibody responses and persistence for CAE virus infection were detected by Western blotting. After CAE virus infection, antibody to gag 28 first appeared between 17 to 39 days postinfection, and then persisted afterward. Following the appearance of antibody to p28, antibodies pl4 and pi 6 appeared later. Both antibodies also persisted in the whole experiment. In contrast, antibody to p135 of goat G3 and G4 appeared on 30 weeks and 10 weeks postinfection, respectively. Goat 1 and 2, sacrified for pathological examination during the early appearance of virus-specifc antibody, did not show typical lesion of the joints except for mild hyperemia with mild lymphocytic infiltration. Goat 4 and 5 sacrified I and 2 years postinfection respectively, showed marked chronic proliferative synovitis with heavy infiltration of mononuclear cells, interstitial pneumonia with thickened alveolar septa with fibrinoid tissue, and extensive lymph follicular hyperplasia of lymph nodes. The negative goat living with CAEV infected goat for two years was still serological negative and did not show any significant pathological lesion. |
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