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題 名 | Modulation of Immune Responses to DNA Vaccines by Codelivery of Cytokine Genes=以細胞激素基因調控基因疫苗引發的免疫反應 |
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作 者 | 潘建雄; 陳信偉; 陶秘華; | 書刊名 | 臺灣醫學會雜誌 |
卷 期 | 98:11 1999.11[民88.11] |
頁 次 | 頁722-729 |
分類號 | 418.293 |
關鍵詞 | 細胞激素; 基因; 基因疫苗; 免疫反應; DNA vaccine; Cytokine; Adjuvant; Antibody; Helper T cell; |
語 文 | 英文(English) |
英文摘要 | DNA vaccines containing genes for antigenic portions of viruses have recently been developed as a novel vaccination technology. Direct injection of plasmid DNA in vivo results in prolonged expression of viral proteins and may, thus, mimic the action of attenuated vaccines. An important advantage of this vaccination method is that in vivo-synthesized viral proteins can enter both major histocompatibility complex (MHC) class I and class II antigen-processing pathways to activate specific immunization. In many animal models for infectious diseases, DNA vaccines induced a broad range of immune responses, including antibody, CD8 �� cytotoxic T lymphocytes (CTL) and CD4 �� helper T (Th) lymphocyte responses, and protective immunity against challenge with the pathogen. The magnitude and nature of these immune responses to DNA vaccines can be further manipulated by codelivery of cytokine genes. Summarizing the many studies reported to date, we can draw conclusions regarding the adjuvant effects of these cytokine genes on DNA vaccines. Coadministration of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-2 genes induces higher antibody titers and T-cell proliferation responses than other cytokine genes tested to date. In contrast, the CTL activity is only modestly increased by the GM-CSF and IL-2 genes. The IL-12 gene polarizes the immune responses to DNA vaccines toward Thl cell development and stimulates the strongest CTL activity. In contrast, co-injection of the IL-4 gene promotes the development of Th2 cells and increases production of antibodies, but suppresses CTL activity. Thus, the immune responses to DNA vaccines can be engineered by co-injection of an appropriate cytokine gene to favor the formation of either CTL or neutralization antibodies and, therefore, provide the best protection against a particular pathogen. |
本系統中英文摘要資訊取自各篇刊載內容。