查詢結果分析
相關文獻
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頁籤選單縮合
題名 | Serological Survey and Virus Isolation of Caprine Arthritis Encephalitis Virus Infection in Taiwan=臺灣山羊關節炎腦炎病毒之血清學調查及病毒分離 |
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作 者 | 李維誠; 韓佳洲; 林正忠; 簡茂盛; 廖永剛; 劉正義; | 書刊名 | 臺灣畜牧獸醫學會會報 |
卷期 | 66:3 1996.09[民85.09] |
頁次 | 頁173-180 |
分類號 | 437.246、437.246 |
關鍵詞 | 山羊關節炎腦炎病; 西方墨點法; 血清學檢查; 病毒分離; Caprine arthritis encephalitis virus; Western blotting; Serology; Virus isolation; |
語文 | 英文(English) |
中文摘要 | 為瞭解山羊關節炎腦炎病在台灣流行情形,從臺灣中南部及東部66個乳羊場所取得的735個血清樣品,以西方墨點法進行血清學之檢查。山羊關節炎腦炎病毒感染之陽性血清可確認綿羊maedi visna病毒之p14、p17、p25及gp110白質其中p25最為套顯且穩定出現,可用為評定山羊關節炎腦炎病看之感染。在此調查中,除臺東2場為陰性場外,餘皆為陽性場。全血清檢查結果平均陽性率為75.9%。病毒可由腫大之關節囊液中於血液單核細胞衍生之巨噬細胞及山羊皮皮膚角質細胞之培養中被分離出來,病毒在兩種培養細胞中均形成多核之融合細胞。此為山羊關節炎腦炎病毒首次在臺灣之分離報告。 |
英文摘要 | To understand to prevalence of caprine arthritis encephalitis virus infection in dairy goats in Taiwan, 735 sera from 66goat farms in central, south and east Taiwan were examined by Western blotting technique using maedi visna virus (MVV) antigen. Although the caprine arthritis encephalitis positive sera recognize MVV p14, p17, p25 (gag proteins) and 110 (env protein) , only antibody to p25 appeared consistently in most positive sera. Prevalence of caprine arthritis encephalitis virus infection among dairy goats was found to be 75.9% (558/735). Viruses could be isolated from synovial fluid from goats with swollen capral joints using goat monocyte-derived-macrophage and skin keratinocyte coultures. Both cell cultures infected with CAEV showed marked syncytial formation. This is the first isolation of CAEV in Taiwan. |
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