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頁籤選單縮合
題名 | Identification of a Measles Virus Isolate from a Recent Outbreak in Northern Taiwan=臺灣麻疹病毒分離株之鑑別 |
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作者姓名(中文) | 關媺媺; 王躬仁; | 書刊名 | Proceedings of the National Science Council : Part B, Life Science |
卷期 | 21:3 1997.07[民86.07] |
頁次 | 頁96-99 |
分類號 | 415.2776 |
關鍵詞 | 臺灣; 麻疹病毒分離株; Measles virus; Local lineage; |
語文 | 英文(English) |
中文摘要 | 我們利用 RT-PCR 增幅臺灣 1994 年麻疹病毒分離株之核膜蛋白質基因, 並於大 腸桿菌轉殖,篩選得到一個特異限制核酸片段,再分析此基因片段與已知資料比較,結果顯 示: 該病毒不具有疫苗衍生株( Moranten and Schwarz vaccine associated strains ) 之序列特性,亦不同於英、美等地之分離株。本 RT-PCR 可作為例行檢定麻疹病毒基因;並 推論該病毒分離株為具有臺灣品系之遺傳特性。 |
英文摘要 | Specimens were collected during a recent outbreak. Those specimens displaying both CPE positive in B95-B lymphocyte cell culture and positive by IFA were checked by a RT-PCR with a specific set of measles virus primers derived from the C-terminal of the nucleoprotein. Such RT-PCR method was found ideal for routine diagnostic purposes. Product from this RT-PCR was treated for plasmid construction before transformed into E. coli. One of those transformed clones, i.e. T94, was further studied for its DNA sequence. Since T94 is found to bear several evident different characteristics from those ever published, we conclude that this isolate is neither a vaccine derived strain nor one of those reported previously with specific amino acid residues, but unique in its own right. This isolate can well be a local lineage of wild measles virus in Taiwan. |
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