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題 名 | Affect of Garlic Components Diallyl Sulfide and Diallyl Disulfide on Arylamine N-Acetyltransferase Activity in Rat Lymphocytes=大蒜成份Diallyl Sulfide和Diallyl Disulfide影響老鼠淋巴球細胞之醯轉移酵素 |
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作 者 | 何蓁蓁; 賴義雄; 蔡輝彥; 鍾景光; | 書刊名 | 中國醫藥學院雜誌 |
卷 期 | 7:3 1998.09[民87.09] |
頁 次 | 頁89-97 |
分類號 | 418.52 |
關鍵詞 | 乙醯轉移酵素; 芳香氨類化合物; 大蒜的成份; N-acetyltransferase; 2-aminofluorene; P-aminobenmzoic acid; Diallyl sulfide and diallyl disulfide; DAS; DADS; |
語 文 | 英文(English) |
中文摘要 | 大蒜的兩個主要成份Diallyl Sulfide(DAS)和Dially Disulfide(DADS),被用來檢測對老鼠淋巴球細胞乙醯轉移酵素的影響,乙醯轉移酵素利用2-aminofluoren和p-aminobenzoic acid作受質用高壓層析儀來檢測其活性.利用兩種檢測系統,一為打破細胞(9000g);另一為整個活細胞.研究結果發現DAS和DADS隨著濃度上升在此兩種檢測系統下抑制此酵素的活性也隨著增加。這篇文章是第一次報導有關大蒜成份能抑制老鼠淋巴球細胞乙醯轉移酵素的活性。 |
英文摘要 | Diallyl sulfide (DAS) and diallyl disulfide (DADS), major components of garlic, were used to determine inhibition of arylamine N-acetyltransferase (NAT) activity in a rat lymphocytes. N-acetyltransferase activity was determied by high pressure liquid chromatography using 2-aminofluorene and p-aminobenzoic acid as substrates. Two assay systems were performed, one with cellular cytosols (9000 g supernatant), the other with intact lymphocytes suspensions. The NAT activity in a rat lymphocytes was inhibited by DAS and DADS in a dose-dependent manner in both systems: i.e., the greater the concentration of DAS and DADS in the reaction, the greater the inhibition of NAT activities in both systems. This report is the first demonstration to show that garlic components affect rat lymphocyte cells NAT activity. |
本系統中英文摘要資訊取自各篇刊載內容。