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題 名 | Effect of Vitamin E on Arylamine N-Acetyltransferase Activity in Colon Cancer Tissues=Vitamin E對人體正常大腸組織及大腸癌組織中乙醯轉移酵素活性的影響 |
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作 者 | 洪啟賦; 鍾景光; 蘇志中; 劉素瑛; 廖仁; 廖美姬; 洪禾佳; | 書刊名 | 放射治療與腫瘤學 |
卷 期 | 6:1 1999.03[民88.03] |
頁 次 | 頁1-7 |
分類號 | 416.245 |
關鍵詞 | 芳香胺化合物; N基乙醯轉移酵素; 大腸癌; 維生素E; Arylamine; N-acetyltransferase; Colon cancer; Vitamin E; |
語 文 | 英文(English) |
中文摘要 | 目的:芳香胺化合物經N基乙醯轉移酵素之N基乙醯化後可誘發大腸癌,本研究是 in vitro 探討正常大腸組織及大腸癌組織中乙醯轉移酵素的活性及 Vitamin E 對正常大腸 組織及大腸癌癌組織中乙醯轉移酵素活性的影響。材料與方法:取 40 個正常大腸組織及大 腸癌組織利用乙醯化輔脢作乙醯基而再利用高壓層析儀分析乙醯轉移酵素的活性及 Vitamin E 對正常大腸組織及大腸癌癌組織中乙醯轉移酵素活性的影響, 以 aminofluorene 和 p-aminobenzoic acid 作受質。結果:結果大腸癌組織乙醯轉移酵素活性較正常組織中乙醯 轉移酵素活性高,Vitamin E 對正常大腸組織及大腸癌癌組織中乙醯轉移酵素的活性有降低 的作用。 結論:Vitamin E 對大腸癌的治療及預防有可能具臨床的價值。本實驗報告是 in vitro 探討的初步報告,當然須再進一步 in vivo 的研究確認。 |
英文摘要 | Purpose:Arylamine N-acetylation capacity by the N-acetyltransferase ( NAT ) may be an important causative factor in the initiation of colorectal cancer. Vitamin E ( alpha-tocopherol ) has been known for many years as an antioxidant and act as an important component of anticancer regiments. The activity of NAT and the effect of vitamin E on NAT activity in healthy colon and colon cancerous tissues ( invitro ) were investigated.Materials and Methods: Forty human healthy colon and colon cancerous tissue cytosol ( subcellular fraction ) were investigated for NAT activity. The effect of vitamin E on the activity of NAT was also examined by high pressure liquid chromatography ( HPLC ) using 2-aminofluorene and p-aminobenzoic acid as substrates in this study. Ressults: NAT activity are higher in colon cancerous tissue cytosol than in healthy colon tissue cytosol vitamin E could decrease NAT activity in healthy colon and colon cancerous tissue cytosol. Conclusion:The results may suggest a clue to the use of vitamin E in treatment and prevention of human colon cancer. In future, in vivo study will be investigated. |
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