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- Arylamine N-Acetyltransferase Activity (NAT) in Rat Glial Tumor Cells
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題 名 | Effect of Carmustine (BCNU) on N-acetyltransferase Activity in Rat Glioma Cells=Carmustine對大白鼠大腦膠質瘤細胞株中乙醯轉移酵素活性的影響 |
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作 者 | 洪啟賦; 鍾景光; 廖仁; 廖美姬; 謝明村; 徐雷鐸; 陳建良; | 書刊名 | 中國醫藥科學雜誌 |
卷 期 | 1:1 2000.03[民89.03] |
頁 次 | 頁35-40 |
分類號 | 414.5 |
關鍵詞 | 芳香胺化合物; N基乙醯轉移酵素; 膠質細胞瘤; Arylamine; N-acetyltransferase; Carmustine; BCNU; Glioma; |
語 文 | 英文(English) |
中文摘要 | 芳香胺化合物經N基乙醯轉移酵素(N-acetyltransferase)之N基乙醯化可 誘發癌,本研究是in vitro探討carmustine(BCNU)對大白鼠大腦膠質瘤細胞株中 乙醯轉移酵素活性(NAT)的影響。取12個大白鼠大腦膠質細胞瘤組織用乙醯化 輔脢A作乙醯基而再利用高壓液相層析儀分析乙醯轉移酵素的活性及BCNU 對 大白鼠大腦膠質細胞瘤組織中乙醯轉移酵素活性的影響, 以2-aminofluorene(2- AF)和p-aminobenzoic acid(PABA)作受質。 結果顯示白鼠大腦膠質瘤細胞株中對AF及PABA受質皆有乙醯轉移酵素的 活性存在,其對AF及PABA的活性平均值各為1.08 ±0.18 及 0.96± 0.16 nmol/min/mg protein。在0.8 mM, 8mM and 80mM的carmustine濃度中,白鼠大 腦膠質瘤細胞株中PABA-NAT活性降低約為16.6%, 31.3% and 50% ,AF-NAT 活性降低約為20.4%, 35.2% and 51.9%。 Carmustine降低白鼠大腦膠質瘤細胞株 中乙醯轉移酵素的活性的作用, 可能是carmustine治療膠質細胞瘤的一種機轉。 本實驗報告是in vitro探討的初步報告,當然須再進一步in vivo的研究確認。 |
英文摘要 | Rat glioma cell cytosol (subcellular fraction) was used to investigate the effect of carmustine (BCNU) on the activity of NAT toward p-aminiobenzoic acid and 2-amniofluorene using an acetyl coenzyme A (AcCoA) recycling assay and high pressure liquid chromatography (HPLC). BCNU is a cancer chemotherapeutic agent used in the treatment of brain tumor. Activity of N-acetyltransferase in rat glioma cell cytosol is found to be inhibited by BCNU. Its presence in the assay medium shows an inhibition in PABA-NAT activity about of 16.6%, 31.3% and 50% in rat glioma cells at 0.8 mM, 8mM and 80mM of BCNU concentrations respectively; while as AF-NAT activity decreased about 20.4%, 35.2% and 51.9% in rat glioma cells with 0.8 mM, 8mM and 80mM of BCNU concentrations respectively. The results of this study show that NAT activity is decreased by BCNU, which can be attributed to its anticancer activity. |
本系統中英文摘要資訊取自各篇刊載內容。