查詢結果分析
來源資料
相關文獻
- Effect of Vitamin E on Arylamine N-Acetyltransferase Activity in Colon Cancer Tissues
- Effect of 5-Fluorouracil on N-Acetyltransferase Activity in Colon Cancer Tissues
- N-Acetyltransferase Activity and 2-Aminofluorene-DNA Adducts Formation in Human Colon Cancer
- 大腸癌手術的現況
- Arylamine N-Acetyltransferase Activity (NAT) in Rat Glial Tumor Cells
- Adenocarcinoma of the Colon Presenting with a Sister Mary Joseph's Nodule (Umbilical Metastasis)--Case Report
- 大腸癌的最近發展
- 多發大腸癌之後視力喪失及右肢無力
- 大腸癌之肝臟轉移的手術治療
- Biliary Papillomatosis and Colon Cancer: A Case Report
頁籤選單縮合
題 名 | Effect of 5-Fluorouracil on N-Acetyltransferase Activity in Colon Cancer Tissues=5-Fluorouracil對大腸癌組織中乙醯轉移酵素活性的影響 |
---|---|
作 者 | 洪啟賦; 鍾景光; 顧國極; 廖仁; | 書刊名 | 中華民國外科醫學會雜誌 |
卷 期 | 32:6 民88.11-12 |
頁 次 | 頁245-251 |
分類號 | 416.245 |
關鍵詞 | 大腸癌; N基乙醯轉移酵素; Arylamine; Colon cancer; 5-fluorouracil; N-acetyltransferase; |
語 文 | 英文(English) |
中文摘要 | 芳香胺化合物經N基乙醯轉移酵素(NAT)之N基乙醯化可誘發大腸癌,本研究是in vitro探討正常大腸黏膜及大腸癌組織中乙醯轉移酵素的活性及5-Fluorouracil (5Fu)對正常大腸黏膜及大腸癌組織中 乙醯轉移酵素活性的影響。取40個正常大腸黏膜及大腸癌組織利用乙醯化輔脢A作乙醯基而再利用高壓層析儀分析乙醯轉移酵素的活性及5-Fu對正常大腸黏膜及大腸癌組織中乙醯轉移酵素活性的影響,以2-aminofluorene(2-AF)和p-aminobenzoic acid(PABA)作受質。 結果常大腸黏膜及大腸癌組織中皆有乙醯轉移酵素的活性存在,大腸癌組織中乙醯轉移酵素活性較正常黏膜組織中乙醯轉移酵素活性高。5-Fu對正常大腸黏膜組織及大腸癌組織中乙醯轉移酵素的活性有降低的作用,在1mM及2mM的5-Fu濃度中,正常大腸黏膜中PABA-NAT活性降低約為23.4%及50.5% (p<0.05) ,大腸癌組織中PABA-NAT活性降低約為48.3%及73.8%(p<0.02) ;而正常大腸黏膜中AF-NAT活性降低約為21.2%及55.1 % (p<0.05) ,大腸癌組織中AF-NAT活性降低約為42.2%及68.4%(p<0.02)。 5-Fu降低正常大腸黏膜及大腸癌癌組織中乙醯轉移酵素的活性的作用,可能是5-Fu治療大腸癌的一種機轉。本實驗報告是in vitro探討的初步報告, 當然須再進一步in vivo的研究確認。 |
英文摘要 | Human epidemiological studies suggest an association between N-acetyltransferase (NAT) and colon cancer. Arylamine N-acetylation capacity of the human colon mucosa, catalyzed by acetyl coenzyme A-dependent N-acetyltransferase, may be an important causative factor in the initiation of colorectal cancer. Human healthy colon mucosa and colon cancerous cytosol(subcellular fraction) from 40 fresh surgical samples were investigated for the effect of 5-fluorouracil (5-FU) on the activity of NAT toward p-aminiobenzoic acid and 2-amniofIuorene using an acetyl cocnzyme A (AcCoA) recycling assay and high pressure liquid chromatography (HPLC). 5-FU is a cancer chemotherapeutic agent used in the treatment of colon cancer. Activity of N-acetyltransferase in healthy colon mucosa and colon cancerous cytosol is found to be inhibited by 5-FU. Its presence in the assay medium shows an inhibition in PABA-NAT activity about of 23.4% and 50.5% in healthy colon mucosa (p<0.05), 48.3% and 73.8% in cancerous colon tissues (p<0.02) at 1mM and 2mM of 5-FU concentrations respectively; while as AF-NAT activity decreased about 21.2% and 55.1% in healthy colon mucosa (p<0.05), 42.2% and 68.4% in cancerous colon tissues (p<0.02) with lmM and 2mM of 5-FU concentrations respectively. The results of this study show that NAT activity in colon cancerous tissue cytosol is higher than that in healthy colon mucosa cytosol, and 5-FU can inhibit NAT activity in healthy colon mucosa as well as in colon cancerous tissue cytosol which can be attributed to its anticancer activity of 5-FU. |
本系統中英文摘要資訊取自各篇刊載內容。