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題名 | 2-Aminofluorene-DNA Adducts formation in Rat Glial Cultured Tumor Cells=大白鼠神經膠質細胞瘤組織中2-AF-DNA的形成 |
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作者 | 蘇志中; 廖美姬; 廖仁; 洪禾佳; 鍾景光; 劉素瑛; 洪啟賦; | 書刊名 | 放射治療與腫瘤學 |
卷期 | 6:3 1999.09[民88.09] |
頁次 | 頁173-177 |
分類號 | 415.138 |
關鍵詞 | 芳香胺化合物; 去氧核糖核酸酶; 神經膠質瘤; Arylamine; DNA; Glioma; |
語文 | 英文(English) |
中文摘要 | 目的:芳香胺化合物經N基乙醯轉移酵素之N基乙醯化後可誘發癌□症,本研究是 in vitro探討大白鼠神經膠質培養腫瘤細胞中2-AF-DNA的形成。 材料與方法:取大白鼠神經膠質培養腫瘤細胞利用乙醯化輔□A作乙醯基及γ-[32p]-dATP而 再利用高壓層析儀分析2-AF-DNA的形成性,以aminofluorene(AF)作受質。 結果:結果大白鼠神經膠質培養腫瘤細胞有乙醯轉移酵素的活性存左,其對濃度30 uM and 60 uM AF為受質形成2-AF-DNA adducts各為0.48±0.16 and 0.70±0.12 pmol/mg DNA。 結論:結果發現大白鼠神經膠質培養腫瘤細胞中乙醯轉移酵素,可將2-AF乙醯化成2-AAF與 細胞中去氧核糖核酸□結合為2-AF-DNA。本研究報告是in vitro探討的初步報告,未來需進 一步以in vivo的實驗確認。 |
英文摘要 | Purpose: Arylamine N-acetylation capacity by the N-acetyltransferase (NAT) may be an important causative factor in the initiation of cancer. Most carcinogens have been demonstrated to react with cellular DNA to form covalent DNA adducts. Arylamine-DNA adducts formation have been correlated with the carcinogenic effect of heterocyclic aromatic amines. 2-AF-DNA formation in rat glial cultured tumor cell line was investigated. Materials and Methods: 2-aminofluorene-DNA (2-AF-DNA) adducts formation in rat glial cultured tumor cell line was investigated by γ-[32p]-dATP and HPLC, using 2-aminofluorene as substrates. Results: 2-AF-DNA adducts formation in rat glial cultured tumor cell with 30 uM and 60 uM AF were 0.48±0.16 and 0.70±0.12 pmol/mg DNA, respectively. Conclusion: The results indicate that rat glial cultured tumor cells activate AF to a metabolite able to bind covalently with DNA, and also induced dose-dependent effect. |
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