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題 名 | Characterization of Allergens of Penicillium and Aspergillus Species=青黴菌與麴黴菌過敏原之特性 |
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作 者 | 沈弘德; 韓韶華; | 書刊名 | 微免與感染雜誌 |
卷 期 | 31:3 1998.09[民87.09] |
頁 次 | 頁141-145 |
分類號 | 414.85 |
關鍵詞 | 青黴菌; 麴黴菌; 過敏原; Allergens; Penicillium sp.; Aspergillus sp.; |
語 文 | 英文(English) |
中文摘要 | 青黴菌與麴黴菌為室內空氣中常見之黴菌,亦被證實為引起外因性氣喘重要原因 之一。但是我們對這些普遍存在於室內環境中之黴菌過敏原特性,了解仍相當有限。由我們 的調查結果顯示,臺北地區空氣中最常見之青黴菌為 P. citrinum。利用過敏病人血清,由 SDS--PAGE-- 免疫轉印分析結果顯示,分子量約 33 kDa 成分為 P. citrinum,P. notatum 以及 P. brevicompactum 之主要過敏原成分;且這些主要過敏原間有 IgE 交互反應存在。 進一步之 N 端氨基酸序列分析結果顯示, P. citrinum 以及 P. brevicompactum 之 33 kDa 主要過敏原成分為各青黴菌種之 alkaline serine proteinase。 此外,我們的結果亦 顯示 A. oryzae 之 34 kDa 之主要過敏原亦為 alkaline serine proteinase; 且 P. citrinum 與 A. oryzae 之主要 serine proteinase 過敏原間有 IgE 交互反應存在。此外 , 經由 cDNA 選殖之研究結果顯示, P. notatum 之 68 kDa 過敏原為β -N-acetyl-glucosaminidase;而熱休克蛋白 70 亦被證實為 P. citrinum 一過敏原成分。 我們所得到的這些研究成果將提供臨床黴菌過敏病之診斷與治療重要參考資訊與根據。 |
英文摘要 | Penicillium and Aspergillus species are common indoor airborne fungi and have been identified to be important causative agents of extrinsic bronchial asthma. However, little was known about allergens of these ubiquitous fungal species. Results from a survey conducted by us showed that P. citrinum was the most prevalent Penicillium species in the Taipei area. Characterization of allergens by SDS-PAGE-immunoblotting using sera from asthmatic patients showed that there was an IgE cross-reactivity among the 33 KDa group major allergens of P. citrinum, P. notatum and P. brevicompactum. Results obtained from N-terminal amino acid sequence analysis suggest that the 33 KDa major allergens of P. citrinum and P. brevicompactum may be the alkaline serine proteinase of individual Penicillium species. In addition, our results suggest that the 34 KDa major allergen of A. oryzae is also an alkaline serine proteinase. IgE cross-reactivity between the major serine proteinase allergens of A. oryzae and P. citrinum has also been detected. Furthermore, results from cDNA cloning suggest that the 68 KDa allergen of P. notatum is β -N-acetyl-glucosaminidase. Lastly, a heat shock protein in the hsp70 family has also been identified as an allergen of P. citrinum. Results obtained in these studies will provide important basis for clinical diagnosis and treatment of mould allergy. |
本系統中英文摘要資訊取自各篇刊載內容。