頁籤選單縮合
題名 | 菊花葉片癒傷組織之培養=The Callus Culture of Chrysanthemum (Dendranthema × Grandiflora (Ramat.) Kitamura) from Leaf Explants |
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作 者 | 江純雅; 黃敏展; | 書刊名 | 興大園藝 |
卷期 | 22:2 1997.12[民86.12] |
頁次 | 頁73-85 |
分類號 | 435.435 |
關鍵詞 | 菊花癒傷組織培養; 再生; 不定芽; Chrysanthemum; Dendranthema × grandiflora; Ramat; Kitamura; Callus culture; Regeneration; Adventitious shoots; |
語文 | 中文(Chinese) |
中文摘要 | 將菊花( Dendranthema × grandiflora(Ramat.) Kitamura )品種, 〞秀芳之 力〞、 〞 Costume 〞、〞 Monami 〞及〞紅小菊〞的葉片培殖體以全量 MS 基本培養基添 加 IAA 5mg/l、BA 10 mg/l 及 2,4-D 1 mg/l 的結果,可透導癒傷組織生成。 〞秀芳之力 〞與〞紅小菊〞癒傷組織在 IAA 10 mg/l 及 BA 10 mg/l 的培養基中增殖情況良好, 且可 再生枝梢。 〞 Costume 〞品種在 BA 1 mg/l 與 IAA 0.5 或 1 mg/l 的培養基配方中,可 形成較為疏鬆的癒傷組織,增殖量大,再分化能力最佳。IAA 易使〞 Monami 〞品種癒傷組 織褐化, 但在 BA 0.5 mg/l 與 NAA 0.05 或 1 mg/l 的培養基中,癒傷組織增殖量最大且 可自然的再生植株。每公升 6 或 8 公克的洋菜較適於癒傷組織增殖,新梢移至不含生長調 節物質的培養基中可促使伸長、發根,而每公升 30 或 20 公克的蔗糖對瓶內植株的生長及 出瓶後之存活率較為有利。 |
英文摘要 | Callus were induced from leaves of DendranthemaXgrandiflora (Ramat.) Kitamura cultivars, such as "Shiu Fang Chih Li", "Costume", "Monami" and "Red Small Type Mum". The factors affected callus proliferation and shoots regeneration were studied. Calli of these 4 cultivars were proliferated from leaf explants cultured on the medium containing basal MS medium, 5 mg/l of indole-3-acetic acid, 10 mg/l of 6-benzyladenine, 1 mg/l of 2,4-dichlorophenoxyacetic acid, 30 g/l of sugar, and solified by 8 g/l Difco Bacto-agar. Callus of "Shiu Fang Chih Li" and "Red Small Type Mum" cultured on the medium containing basal MS medium, 30 g/l of sugar, 10 mg/l of indole-3-acetic acid, 10 mg/l of 6-benzyladenine and solified by 6 g/l Difco Bacto-agar developed into adventitious shoots and more calli. Callus of "Costume" cultured on basal MS medium, 0.5 or 1 mg/l of indole-3-acetic acid, 1 mg/l of 6-benzyladenine, developed more fibrous callus and more shoots. Callus growth of "Monami" were limited and browning by increasing indole-3-acetic acid concentratinos. The callus growth and shoots formation of "Monami" were promoted when cultured on the medium containing 0.05 or 0.1 mg/l of 1-naphthaleneacetic acid and 0.5 mg/l of 6-benzyladenine. Callus growth was also promoted when it was cultured on a medium solified by 6 or 8 g/l agar, medium without plant growth regulators accelerated shoots elongation and root initiated. Plantlets cultured on the basal MS medium containing 30 or 20 g/l of sucrose was favorable for growth in vitro and survival ex vitro. |
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