查詢結果分析
來源資料
頁籤選單縮合
題名 | Thidiazuron及2,4-D或Picloram促進火鶴花試管苗葉片之培養再生不定芽=Thidiazuron Promotes Adventitious Shoot Regeneration on 2,4-D or Picloram Containing Medium in in vitro Lamina Culture of Anthurium |
---|---|
作者 | 楊苑欣; 林金和; 陳福旗; Yang, Yuan-hsin; Lin, Chih Ho; Chen, Fure-chyi; |
期刊 | 中國園藝 |
出版日期 | 20031200 |
卷期 | 49:4 2003.12[民92.12] |
頁次 | 頁375-381 |
分類號 | 435.46 |
語文 | chi |
關鍵詞 | 火鶴花; 葉片培養; 不定芽再生; Anthurium; Lamina culture; Adventitious shoot regeneration; |
中文摘要 | 火鶴花Anthurium sp. T2營養系試管苗葉片,以半量之Murashige及Skoog配方(1/2 MS)為基本培養基,單獨添加生長素Picloram或2,4-D不易再生芽體,但有少量的癒合組織形成;添加0.5 mg/l TDZ於2,4-D(0~0.5 mg/l)培養基,不定芽形成率介於10~30%;不論在1/2 MS或MS培養基,添加TDZ均能誘導癒合組織形成。T18營養系試管苗葉片培養於以全量MS及0.5 mg/l TDZ為基本培養基,添加Picloram之處理,易形成癒合組織,而不定芽形成率均較含單獨TDZ者低;再生之植株其外觀正常。 |
英文摘要 | A method of plant regeneration from in vitro lamina culture of Anthurium by supplementing thidiazuron with either 2,4-D or picloram is examined. Whole leaf blade of T2 lamina produced few callus on 1/2 MS medium supplemented with 2,4-D or picloram. A combination of 2,4-D (0.1~1mg/l) and TDZ (0.5mg/l) led to 10~30% of the explants regenerating adventitious shoots. MS medium supplemented with 0.5 mg/l TDZ and picloram (0.1~1 mg/l) induced 0~70% shoot formation in T18 lamina. Reducing salt strength to one half promoted callus formation. Normal and rooted plantlets were recovered and subcultured on the same medium. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。