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題名 | 以薄層色層掃描儀分析靈芝子實體三萜類模式區別赤芝及松杉靈芝之研究= |
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作者 | 賴敏男; 楊依珍; 蘇慶華; 董一致; |
期刊 | 嘉義農專學報 |
出版日期 | 19950800 |
卷期 | 42 1995.08[民84.08] |
頁次 | 頁51-62 |
分類號 | 414.33 |
語文 | chi |
關鍵詞 | 薄層色層分析; 赤芝; 松杉靈芝; 三萜類; Thin-layer chromatography; Ganoderma lucidum; Ganoderma tsugae; Triterpenoids; |
中文摘要 | 本研究嚐試利用TLC及TLC掃描儀分析靈芝子實體三�I類之分佈情形, 以建立不同靈芝種之三�I類模式,探討作為鑑別靈芝種之可行性。在不同地區農 場採集特性極相似赤芝(Ganoderma lucidum)及松杉靈芝(Ganoderma tsugae)等兩種 菌株栽培所得之各種不同形狀子實體作為試樣,以TLC掃描儀分析出之12種三�I 類圖譜模式可歸納二種型式,而此二種型式與以掃描式電子顯微鏡觀察出之靈芝 孢子形態作比對,兩者對上述二種菌種特性之描述相當一致。另外選擇赤芝 (G.lucidum N0.4)同一種菌抹在不同栽培條件所得9種子實體,其所分析出之每一 種子實體三�I類圖譜構造都相同。綜合上述之分析結果證明不同種靈芝所含三�I 類有一定模式 ,不受子實體形狀、色澤及栽培條件等因素之影響,因此利用TLC 及TLC掃描儀分析不同種靈芝子實體所含三�I類之圖譜模式可作為靈芝種分類 或鑑別市售靈芝製品品質之參考。 |
英文摘要 | In order to establish a model for identification of the genus Ganoderma,thin layer chromatography scanner were used to study the triterpenoidpatterns. Ganoderma lucidum and Ganoderma tsugae, both have similar morphological characteristics were selected for this study. Fruiting bodysamples with different forms obtained from cultivating farms were used.Results show that the twelve triterpenoid patterns obtained from twelvesamples can be classified into two groups .Using the scanning electron microscopy to compare the basidospore morphology of these two groups,consistent to the triterpenoid pattern classification , a similar characteristics as described by Adaskaveg and Gilbertson was observed. It is also evident that thetriterpenoid patterns of 9 fruiting bodies from the same strain cultures of G.lucidum N0.4 under different cultivating condition were almost identical.This indicates that there is a standard triterpenoid pattern between the different Ganoderma species , and it is not affected by the form , color andcultivating conditions . Therefore , it is feasible to use the thin layerchromatography scanner to identify triterpeniod patterns of the Ganodermafruiting bodies.as well as using this patterns to classify the genusGanoderma and to evaluate the quality of Ganoderma products. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。