查詢結果分析
相關文獻
- Determination of Ganoderic Acids in Triterpenoid Constituents of Ganoderma Tsugae
- 以薄層色層掃描儀分析靈芝子實體三萜類模式區別赤芝及松杉靈芝之研究
- 利用薄層色層掃描儀對不同種靈芝之三萜類鑑定模式之可行性
- 靈芝生物活性成分與生物活性之療養品觀
- Activation of Alternative Complement Pathway by a β-1,6;1,3-D-Glucan from Ganoderma tsugae
- Application of PCR-Amplified DNA to Differentiate the Ganoderma Isolates
- Ganoderic Acid Derivatives, 3-Oxo-Lanostane-Type Triterpenoids, from Ganoderma Lucidum
- 松杉靈芝之栽培及其菌絲之深層培養
- 靈芝太空包多切口栽培法
- 松杉靈芝中類三萜化合物定量方法
頁籤選單縮合
題名 | Determination of Ganoderic Acids in Triterpenoid Constituents of Ganoderma Tsugae=松杉靈芝三萜類成分靈芝酸之定量 |
---|---|
作者 | 陳登海; 陳光地; Chen, Deng-hai; Chen, William Kuan-dee; |
期刊 | 藥物食品分析 |
出版日期 | 20030900 |
卷期 | 11:3 2003.09[民92.09] |
頁次 | 頁195-200+267 |
分類號 | 414.33 |
語文 | eng |
關鍵詞 | 靈芝; 松杉靈芝; 三萜類; 靈芝酸; Ganoderma; Ganoderma tsugae; Triterpenoid; Ganoderic acid; |
中文摘要 | 靈芝產品為國內保健食品之大宗,松杉靈芝 (Ganoderma tsugae) 子實體是最常使用的原料品種。本研究自松杉靈芝子實體中分離出九種三菇類 (triterpenoids),這些三菇類經氫核核磁共振化學位移,紅外線光譜圖在質譜鑑定後,確認為靈芝酸 (ganoderic acid) A (1),B (2),C (3),D (4),E (5),C5 (6),C6 (7),G (8)及靈芝烯酸D (9) (ganoderenic acid D),本研究並建立以逆相高效液相層析 (reverse-phase HPLC) 法,分析松杉靈芝原料中此九種三類的含量、其中靈芝酸E,C5,C6,G及靈芝烯酸D首次以HPLC分離定量。 本實驗之高技液相層析法係採用CI8逆相層折管柱,以梯度沖提方式分離,移動相為不同比例之乙腈;2%醋酸 (1:2及1:4,體積比),檢測波長為252nm,流速為0.8mL/min,得到良好分析結果。九種靈芝酸之線性回歸方程式之相關係數 (r) 皆大於0.9990 (n=5),添加試驗回收率為96.85%-105.09%。同日內標準偏差為0.8-4.8%,異日間標準偏差為0.5-5.1%。以此九種三菇類總含量計算,利用上述液相層析法,檢測市售六種靈之產品及二種松杉靈芝原料,其靈芝酸總含量為0.28%-2.2%。 |
英文摘要 | Ganoderma product has the highest sale volume in the health food market in Taiwan. The most widely-used raw material is the species of G. tsuage. Nine triterpenoids were isolated from the fruiting body of G. tsugae and their structures were identified, using 1H NMR and FT-IR, as ganoderic acid A (1), B (2), C (3), D (4), E (5), C5 (6), C6 (7), G (8) and ganoderenic acid D (9). A reverse-phase HPLC method was also established for the determination of these nine triterpenoids. Among these compounds, ganoderic acid E, C5, C6, G and ganoderenic acid D were analyzed for the first time by reverse-phase HPLC. The separation was carried out on a C18 reverse-phase column with a gradient elution of acetonitrile and 2% acetic acid (1/4 and 1/2, v/v) as mobile phase at a flow-rate of 0.8 mL/min and detection at 252 nm. Regression equation revealed the linear relationship (correlation coefficient: 0.9990~0.9999) between the peak-area ratios and concentrations of ganoderic acids. The recoveries of nine ganoderic acids were between 96.85~105.09%. The relative standard deviations of nine ganoderic acids range from 0.8~4.8% (intraday) and 0.7~5.1% (interday). The total contents of the nine ganoderic acids in eight Ganoderma samples were between 0.28~2.20%. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。