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題名 | Activation of Alternative Complement Pathway by a β-1,6;1,3-D-Glucan from Ganoderma tsugae=松杉靈芝中分離出具活化補體替代路徑之β-1,6;1,3-D-Glucan |
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作者 | 李明彥; 王西華; Lee, Min-yen; Wang, Hsi-hua; |
期刊 | 大仁學報 |
出版日期 | 20000500 |
卷期 | 18 2000.05[民89.05] |
頁次 | 頁43-55 |
分類號 | 379.14 |
語文 | eng |
關鍵詞 | 松杉靈芝; 成份分析; Polysaccharide; β-1,6;1,3-D-glucan; Ganoderma tsugae; Broth; Chemical structure; Complement; |
中文摘要 | 本研究將松杉靈芝培養十四天後之醱酵液以75%酒精將多醣沉澱出來,再以酸水解,經高效能層析儀證明其組成份為葡萄糖,再經甲基化、氣液相層析質譜儀、13C核磁共振儀證明其化學結構是以β-1, 3-D-glucan為主鏈,主鏈平均每三個葡萄糖即有一個β-1,6的支鏈,此支鏈長度為一個葡萄糖分子;經由膠體層析檢定其分子量為219,500。最後並以體外之溶血實驗證明松杉靈芝的β-1,6;1,3-D-Glucan具有活化補體替代路徑的能力,而補體的活化被視為β-1, 6; 1, 3-D-glucan可能增強人體免疫作用機制的起始作用之一。 |
英文摘要 | An extracellular polysaccharide was prepared by the precipitation from the broth of Ganoderma tsugae with ethanol after 14 days of shaking culture. Only glucose was found in the acid hydrolysate of the polysaccharide by HPLC. The polysaccharide was submitted to methylation GC-Mass and 13C-NMR analysis and was identified as a β-1, 3-D-glucan having one branch at every third main chain glucosyl unit each C-6 position, and the length of the branch was one glucose unit. The molecular weight of polysaccharide was 219,500, which was analyzed by fractogel HW-65(s). an activation of human alternative complement pathway by the β-1, 6; 1, 3-D-glucan of G. tsugae was proved by haemolytic analysis. The alternative complement pathway was assumed to be important in the first step of the host defense mechanism. |
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