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| 題 名 | Effect of Cobalt-60 Irradiation on Osteogenesis=鈷六十放射線照射對骨生成的影響 |
|---|---|
| 作 者 | 許文林; 史中; 王天美; 吳錦榕; 黃經民; | 書刊名 | 醫學研究 |
| 卷 期 | 15:3 1994.11[民83.11] |
| 頁 次 | 頁191-210 |
| 分類號 | 414.93 |
| 關鍵詞 | 骨生成; 照射; 原始造骨細胞; 造骨細胞; Osteogenesis; Irradiation; Osteoprogenitor cells; Osteoblast; |
| 語 文 | 英文(English) |
| 中文摘要 | 維護骨組織及細胞的健全結構及功能是臨床醫學上重要及必須深入探討的課題。鈷六十放射線可殺死腫瘤及正常細胞(包括骨髓細胞和骨中細胞)而導致組織不同程度的損傷。本實驗的目的係利用細胞培養法,骨組織形態計量法,光源及電子顯微鏡學來觀察骨髓細胞,骨組織、細胞及骨生成在鈷六十照射後的定性及定量反應。實驗方法係使用60隻約100公克重Sprague-Dawley雄性大白鼠,按隨機抽樣法分成四組(其中一組為對照組)。各組實驗組(施予100,400及1600雷得的鈷六十射線照射)在照射1,2及5天後分別犧牲之。實驗結果。發現大白鼠在鈷六十放射線照射後,其長骨(兩側股骨)中小比重骨髓細胞(含骨原細胞)的數目有顯著減少的現象,並且隨著照射劑量的增加而更明顯。此外,鈷六十照射後會顯著減少骨群體形成的數目和大小。本研究結果顯示,鈷六十放射線照射可以:(1)減少骨髓中骨原細胞的數目,(2)降低造骨原始細胞的分化與造骨細胞的活性,和(3)抑制骨生成的進行。 |
| 英文摘要 | The photon beam from Cobalt-60 can kill both neoplastic and normal cells (including bone marrow and bone cells) and result in various damage of tissues, especially inducing growth retardation of bone of young animals and osteoporotidc fracture of adult animals. The purpose of this study was to investigate the qualitative and quantitative response of bone cells and osteogenesis to Co-60 irradiation by using the in vitro rat bone marrow culture system, bone histomorphometry, light and electron microscopy. Sixty Sprague-Dawley male rats were randomly assigned into 4 groups; every group was irradiated 0, 100, 400 or 1600 rad of irradiation and sacrificed 1, 2 and 5 days after irradiation. With the Ficoll-Paque density gradient separation method, light density (LD) bone marrow white cells were collected and counted. There was a significant decrease in number of LD bone marrow white cells with an increase in dosage of Coblat-60. In addition, ten days after adding LD marrow cells, the number and size of bone colonies formed in the irradiated group was significantly decressed as compared to that in the control. The results of this study indicated that Cobalt-60 irradiation could: 1) decrease the number of bone marrow cells (especially light density bone marrow white cells, 2) reduce the differentiation of osteoprogenitor cells and the activity of osteoblast and 3) inhibit the osteogenesis. |
本系統中英文摘要資訊取自各篇刊載內容。