查詢結果分析
相關文獻
- 第六型介白質對體外骨生成的影響
- Effect of Oncostatin-M on Proliferation and Activity in Osteoblastic MC3T3-E1 Cells
- 第六型介白質對造骨細胞分化的影響
- Effect of Cobalt-60 Irradiation on Osteogenesis
- 移植骨生成細胞預防年齡增長後骨質流失效果及機轉的骨組織形態學研究
- 第一型介白質受體對抗劑和腫瘤壞死因子結合蛋白對去卵巢後骨質減少的效果
- Effects of Cobalt-60 Irradiation on Trabecular Bone
- 牙釉基質蛋白:由生物觀點來探討牙周組織再生及病例報告
- 高壓氧治療對骨生成增強的作用
- 大白鼠骨性肉瘤細胞的造骨細胞表型之研究
頁籤選單縮合
| 題 名 | Effect of Oncostatin-M on Proliferation and Activity in Osteoblastic MC3T3-E1 Cells=Oncostatin-M對造骨細胞株MC3T3-E1細胞增生和活性的影響 |
|---|---|
| 作 者 | 史中; 顏全成; | 書刊名 | 中華醫學雜誌 |
| 卷 期 | 62:10 1999.10[民88.10] |
| 頁 次 | 頁710-716 |
| 分類號 | 415.148 |
| 關鍵詞 | 骨生成; 造骨細胞; 造骨原始細胞; Bone formation; Oncostatin-M; Osteoblast; Osteoprogenitor cell; |
| 語 文 | 英文(English) |
| 中文摘要 | 背景 骨生成細胞(造骨原始細胞和造骨細胞)受到特定細胞激素、生長因子和 荷爾蒙的影響,瞭解何種細胞激素會影響骨生成細胞,有助於闡明正常與病理性骨再塑造過 程。Oncostatin-M(OSM)是一種醣蛋白,屬於第六型介白質類型之細胞激素,在胎兒的長 骨培養中,顯示具有抑制骨吸收的作用。但是,OSM對骨生成是否有影響,則仍不十分清 楚。 方法 利用體外骨生成細胞培養模式,將MC3T3-E1細胞植入35mm培養皿中,並以 DMEM進行培養。在特定培養期間,實驗組培養皿每天加入不同濃度(1、10、100或1,000ng ╱ml)的OSM,而對照組則只使用DMEM。最後,使用放射性 標定胸腺嘧啶嵌入法、鹼 性磷酸 活性、第I型膠原蛋白合成評估法及相位差光源鏡檢,分析OSM對骨生成的影響。 結果 OSM明顯地降低造骨原始細胞的增生和鹼性磷酸 的活性,並且隨著處理濃度 的增加,降低趨勢更顯著;但是OSM對第I型膠原蛋白的合成,並沒有明顯的影響。因此, OSM可顯著地抑制骨生成。 結論 OSM可經由降低造骨原始細胞的增生和鹼性磷酸 的活性,而抑制骨生成。 |
| 英文摘要 | Background. Osteogenic cells (osteoprogenitor cells and osteoblasts) respond to specific cytokines, growth factors and hormones. Understandking which cytokines affect osteogenic cells and the consequences of those effects are central to understanding normal and pathologic bone remodeling. Oncostatin-M (OSM) is a glycorprotein interleukin-6 cytokine known to inhibit bone resorption in fetal long bone cultures. However, it is still unclear whether OSM affects bone formation. The aim of this study was to investigagate the effects of OSM on bone formation (bone cell proliferation, differentiation and function.) Methods. For the in vitro bone formation bioassay, MC3T2-E1 cells were plated into 35 mm Petri dishes and cultured in Dulbecco's modified eagle medium (DMEM). Various concentrations (1, 10, 100 or 1,000 units/ml) of OSM were added daily to the experimental dishes, while only DMEM was added in the control disheds during the proliferative and differentiated stages of MC3T3-E1 cell growth. The effect of OSM on bone formation was evaluated using H-thymidine incorporation, alkaline phosphatase expression, type I collagen synthesis assay and phase-contrast light microscopy. Results. OSM significantly decreased osteoprogenitor cell proliferation and alkaline phosphatase activity in a dose-related fashion. There was no effect on type I collagen synthesis noted at any OSM dose. Thus, OSM exerted significant inhibitoin of bone formation. Conclusions. OSM is a potent inhibitor of bone formation by decreasing both osteoprogenitor cell proliferation and alkaline phosphatase activity. |
本系統中英文摘要資訊取自各篇刊載內容。