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題 名 | 第一型介白質受體對抗劑和腫瘤壞死因子結合蛋白對去卵巢後骨質減少的效果=Effects of Interleukin-1 Receptor Antagonist and Tumor Necrosis Factor Binding Protein on Postovariectomy Osteopenia |
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作 者 | 史中; | 書刊名 | 中華牙醫學雜誌 |
卷 期 | 17:4 1998.12[民87.12] |
頁 次 | 頁242-252 |
分類號 | 415.596 |
關鍵詞 | 第一型介白質受體對抗劑; 破骨細胞; 骨生成; 停經後骨質疏鬆症; 腫瘤壞死因子結合蛋白; Interleukin-1 receptor antagonist; Osteoclast; Osteogenesis; Postmenopausal osteoporosis; Tumor necrosis factor binding protein; |
語 文 | 中文(Chinese) |
中文摘要 | 骨質疏鬆症乃是骨組織中單位體積之骨量減少,當受到小的傷害即易導致骨折。 停經後骨質疏鬆症的產生, 與第一型介自質 (interle 此 in-1,IL-1) 和腫瘤壞死因子 (tumor necrosis factor,TNF)- α的分泌量有極密切的關係。 婦女停經後,IL-1 和 TNF- α分泌量有顯著地增加,而分泌量的增加可刺激骨吸收的進行和造成骨質大量的流失。目前 已知 IL-1 和 TNF- α均能刺激破骨細胞的骨吸收作用和抑制骨生成的進行,但去卵巢或停 經後所引發雌激素缺乏,造成 IL-1 及 TNF- α大量分泌和導致骨質大量流失是否可藉由阻 斷 (block)IL-1 及 TNF- α的作用來有效地防止骨質流失及增加骨生成則不清楚。 因此, 本研究旨在探討第一型介白質受體對抗劑 (IL-1receptorantagonist' IL-1 ra) 及腫瘤壞 死因子結合蛋自 (TNF-binding protein,TNFbp) 對骨生成和骨吸收的影響及預防骨質減少 的效果。 實驗過程中, 利用細胞培養、骨組織形態學和光源鏡檢來評估 IL-1ra 和 TNFbp 對破骨細胞形成的影響。另外,使用膠原酵素分離法,細胞培養,von Kossa 染色法,鹼性 磷酸�t測定法,�� H-thymidine 嵌入法, 膠原蛋自合成測定法,骨組織形態計量法和光源 鏡檢來分析 IL-1 ra 及 TNFbp 對骨生成的影響。結果發現大自鼠去卵巢後,骨群體形成、 造骨原始細胞增生和造骨細胞鹼性磷酸�t活性等骨生成參數和破骨細胞的形成均有增加的情 形。 去卵巢後經 IL-1ra 和 TNFbp 處理後,骨生成各參數有明顯地增加,而破骨細胞形成 的數目則有明顯地減少 (與卵巢切除組比較 )。 因此,IL-1 ra 和 TNFbp 並用可以增加骨 生成和減少破骨細胞數目,並且可能在預防去卵巢後骨質流失上扮演重要的角色。 |
英文摘要 | Osteoporosis is a disease with less bone tissue than normal and predisposes to fracture with minimal trauma. Postmenopausal osteoporosis has been shown to be closely related to the secretion of interleukin-1 (IL-1) and tumor necrosis factor (TNF) - α. Recent evidence suggests that estrogen deficiency after ovariectomy or menopause can cause significant increases in the secretion of IL-1 and TNF - α, and lead to dramatic bone loss. Therefore, blocking the binding of IL-1 and TNF to their receptors could potentially prevent estrogen-deficiency-induced bone loss. IL-1 receptor antagonist (IL-1 ra) binds with IL-1 receptors and competes with both IL-1 α and β without detectable IL-1 agonistic effects. TNF binding protein (TNFbp) binds with TNF tightly to decrease the binding of TNF to its receptor. Thus, the aims of this study were to investigate the effects of IL-Ira and TNFbp on bone formation and osteocalst formation using an in vitro bone marrow culture system and osteoclast formation assay. The effects of IL-Ira and TNFbp on osteogenesis and bone resorption and prevention of bone loss were evaluated using cell culture, von Kossa stain, alkaline phosphatase assay, �� H-thymidine incorporation, collagen synthesis assay, bone histomorphometry and light microscopy. After ovariectomy, the number of bone colonies, bone cell proliferation and alkaline phosphatase activity were increased as compared to controls. After treating with IL-1 ra and TNFbp, bone mass was significantly increased by stimulating bone colony formation, bone cell proliferation and osteoblastic function, and blocking osteoclastic bone resorption. Therefore, IL-1 ra and TNFbp potentially increase bone mass and prevent bone loss postovariectomy. |
本系統中英文摘要資訊取自各篇刊載內容。