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題 名 | Interleukin-1α Stimulates Osteoclast Formation from Peripheral Blood Monocytes and Increases Osteoclastic Activity=第一型介白質-α刺激周邊血液單核球形成破骨細胞和增加破骨細胞的活性 |
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作 者 | 史中; 黃美月; | 書刊名 | 中華醫學雜誌 |
卷 期 | 57:2 1996.02[民85.02] |
頁 次 | 頁85-92 |
分類號 | 415.114 |
關鍵詞 | 骨吸收; 第一型介白質-α; 單核球; 破骨細胞; 停經後骨質疏鬆症; Bone resorption; Interleukin-1α; Monocyte; Osteoclast; Postmenopausal osteoporosis; |
語 文 | 英文(English) |
中文摘要 | 背景 第一型介白質(Interleukin-1,IL-l)可由單核球及骨髓中基質細胞 分泌,其可刺激骨吸收作用的進行。另外,在骨髓細胞長期培養過程中加入IL- 1可誘導類破骨細胞的形成。但IL-1是否可經由刺激周邊血液單核球的融合作 用而促進破骨細胞的形成,一直未有文獻報導。又IL-1對破骨細胞活性的真正 的影響還不清楚。 方法 根據體外破骨細胞形成的模式,自懷孕14天的Sprague-Dawley種大白鼠 中取得不含破骨細胞的胎鼠頭蓋骨組織,然後放入含有BGJb培養液的培養皿 中培養10天。再使用Ficoll-Paque比重梯度分離法獲得周邊血液單核球並加入 培養皿中,同時在實驗組的培養皿中加入分級劑量(1,10或100U/m1)的IL-1 alpha。另外,經由破骨細胞平均含核數計量法和45Ca釋出評估法了解IL-1 alpha 對破骨細胞活性的實際影響情形。 結果 在單核球加入l0天後,發現對照組有14.25個破骨細胞形成,而在實驗 組以分級劑量(1,10或100U/ml)的IL-1alpha處理後,則分別有14.75,18.00或20.75 個破骨細胞形成。另外,IL-1alpha刺激45Ca的釋出和增加破骨細胞平均含核數 並且隨劑量的增加有明顯增加的趨勢,此顯示IL-1alpha可增加破骨細胞的骨吸 收作用。 結論 IL-1alpha可經由刺激周邊血液單核球的融合作用而促進破骨細胞的形 成,並且其可顯著地刺激破骨細胞的活性。 |
英文摘要 | Background. Interleukin-1 (IL-1) is produced by monocytes and marrow stromal cells and can stimulate bone resorption in tissue culture. In addition, IL-1 can induce osteoclast-like cell formation in long-term bone marrow culture. However, whether IL-1 can increase osteoclast formation by stimulating fusion of peripheral blood monocytes is not clear. In addition, the precise effect of IL-1 on osteoclastic activity is still not well known. Methods. Using the bioassay of osteoclast formation, osteoclast-free fetal calvariae were harvested from 14-day timed-pregnant Sprague-Dawley rats and cultured in BGJb medium for 10 days. On the 10th day, mononuclear cells were obtained from peripheral blood of 8-week-old female Sprague-Dawley rats through cardiac puncture and Ficoll-Paque density gradient separation method and co-cultured with osteoclast-free developing bone explant. On the same day, various concentrations of IL-1 alpha (1, 10 or 100 U/ml) were added daily in the experimental dishes while only BGJb medium was added in the controls. In addition, 45Ca release assay and quantitation of nuclei per osteoclast were performed to evaluate the effect of IL-1 alpha on osteoclastic activity. Results. Ten days after adding monocytes, there were 14.25 osteoclasts in the control, without IL-1 alpha; with 1 U of IL-1 alpha, there were 14.75 osteoclasts; with 10 U of IL-1 alpha, there were 18.00 osteoclasts; with 100 U of IL-1 alpha, there were 20.75 osteoclasts. In addition, IL-1 alpha stimulated the release of 45Ca and increased the number of average nuclei per osteoclast dose-dependently, indicating a significant increase in bone resorption. Conclusions. IL-1 alpha could increase osteoclast formation by stimulating the fusion of peripheral blood monocytes. In addition, IL-1 alpha is a potent stimulator of osteoclastic activity. |
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