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題名 | Evaluation of In Vitro Antioxidant and Anti-Lipid Peroxidation Activities of Ching-Pien-Tsao (Pteris Multifida Poiret)=井邊草體外抗氧化與抗脂質過氧化活性之評估 |
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作 者 | 王子慶; 李後易; 楊季清; | 書刊名 | 臺灣農業研究 |
卷期 | 58:1 2009.03[民98.03] |
頁次 | 頁55-60 |
分類號 | 434.92 |
關鍵詞 | 井邊草; 抗氧化; 抗脂質過氧化活性; Pteris multifida poiret; Antioxidant; Anti-lipid peroxidation activity; |
語文 | 英文(English) |
中文摘要 | 本研究藉由硫氰酸鐵法、清除2, 2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS(上標 ˙+))、超氧陰離子、螯合銅離子與抑制FeCl2/H2O2所誘導之亞麻油酸過氧化等方法,評估井邊草水萃取物(aqueous extract of P. multifida Poiret, AEPM)體外總抗氧化能力(total antioxidant capacity, TAC)與抗脂質過氧化活性。結果顯示,AEPM具有高TAC(在20mg/mL濃度下之TAC爲86.7%)與最高之水溶性維他命E(Trolox)等價抗氧化能力(在10mg/mL濃度下爲1.42mM)。AEPM濃度爲20mg/mL時,清除超氧陰離子與螯合銅離子之能力分別爲95.4%及44.7%,亞麻油酸脂質過氧化抑制率則會隨著濃度之增加而增加,其IC50是1000±42μg/mL。 |
英文摘要 | The total antioxidant capacity (TAC), scavenging ability on 2,2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) and superoxide anions, chelating effect on copper ions, and anti FeCl2/H2O2-stimulated linoleic acid peroxidation of aqueous extract of Pteris multifida Poiret (AEPM), were determined in vitro. Results indicated that the AEPM showed a high TAC (86.7% at 20 mg/mL) with ferric thiocyanate method and exhibited the highest Trolox equivalent antioxidant capacity (1.42 mM at 10mg/mL). The scavenging ability on superoxide anion and chelating effect on copper ions of AEPM were 95.4% and 44.7% at 20mg/mL, respectively. The linoleic acid peroxidation inhibition percentage was increased with increasing concentrations and the IC50 was 1000±42μg/mL. |
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