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頁籤選單縮合
題名 | 欖仁葉之抗遺傳毒性研究=Studies on the Antigenotoxic Effect of the Leaf of Terminalia Catappa L. |
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作者 | 李志恆; |
期刊 | 中醫藥年報 |
出版日期 | 19990500 |
卷期 | 17:3 1999.05[民88.05] |
頁次 | 頁347-368 |
分類號 | 418.5 |
語文 | chi |
關鍵詞 | 欖仁葉; 基因突變; 抗氧化物; Terminalia catappa l.; Gene mutation; Antioxidant; |
中文摘要 | 近年來在臺灣相傳用乾枯的欖仁樹落葉煎水做茶飲,或與茶葉 混用沖泡開水服用,有治療肝病的效果。而在最近我們的研究發 現,欖仁葉的水萃取物(TCE)可以預防DNA的被傷害。因此,在本 實驗中將更進一步研究欖仁葉能保護哪一類的致突變劑對DNA的傷 害,並研究其機轉。 本研究使用二種突變劑:烷化劑ethy1 methanesulfonate (EMS), 自由基產生物bleomycin,及需要被cytochromeP-450活化的多環碳 氫化合物3-methylcholanthrene來誘發CHO-K1細胞中hgprt基因突變 並觀察TCE之抗突變能力。結果發現,TCE在低濃度時可以預防 bleomycin所誘發的基因突變,並且可以有限度的部份預防EMS所誘 發的基因突變。但TCE對3-methylcholanthrene則無抗突變性。此 外,TCE也觀察到可以保護bleomycin所造成的DNA斷裂現象及降低 細胞內的過氧化物含量。而TCE成份中含量較高的punicalagin,也 發現具有保護bleomycin所造的DNA斷裂及降低細胞內的過氧化物 含量的能力,但其抗突變能力則較TCE差。由於接受TCE處理過後 細胞內的抗氧化物質,如glutathione含量,catalase、glutathione peroxidase及glutathione transferase的活性皆無明顯變化,所以TCE本 身可能就是抗氧化物,可以去除bleomycin所產生的自由基並避免遺 傳毒性的產生。 本研究中發現TCE對過氧化物所產生的基因毒性具有抗性,但 僅在低劑量範圍的處理下觀察得到,高劑量範圍的處理不僅抗性較 差外,若再提高劑量則更會對細胞造成毒性,因欖仁此茶的服用必 須適量,過多反而有害,這是值得注意並且要進一步探討的問題。 |
英文摘要 | The antigenotoxic effects of T. catappa water extracct(TCE)against hgprt gene mutations, induced by three different types of mutagens, ethy1 methanesulfonate (EMS), 3-methylcholanthrene(3MCA) and bleomycin, were determined in cultured Chinese hamster ovary (CHO) cells. The pretreatments of CHO cells withlower doses of TCE could partially prevent EMS-inducedd hgprt gene mutation and almost prevent completely bleomycin-induced gene mutation while there were no effects on 3MCA-induced gene mutation. in addition, TCE could prevent bleomycin induced c, which plays an important role in bleomycin-induced gene mutation. However, pretreatment of TCE did not elevate glutathione level nor glutathione peroxidase, catalase and gluathione transferase activities in CHO-K1 cells, punicalagin, one of the components of TCE, could partially protect bleomycin-induced gene mutation, DNA damage and intracellular free radical generation. These results demonstrate that TCE can prevent mutagens-induced gene mutations, especially most effective in bleomycin-induced mutation. The antimutagenic activity of TCE against bleomycin induced mutation was possibly due to its antioxidative potential. |
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