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題 名 | Effects of Zinc Deficiency on Endogenous Antioxidant Enzymes and Lipid Peroxidation in Glomerular Cells of Normal and Five-Sixths Nephrectomized Rats=鋅缺乏對於正常和慢性腎衰竭大白鼠腎絲球細胞內抗氧化酵素活性和脂質過氧化作用的影響 |
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作 者 | 陳世明; 楊志剛; | 書刊名 | 臺灣醫學會雜誌 |
卷 期 | 97:11 1998.11[民87.11] |
頁 次 | 頁750-756 |
分類號 | 415.815 |
關鍵詞 | 鋅; 腎衰竭; 腎絲球細胞; 抗氧化酵素活性; 脂質過氧化; Zinc; Antioxidant enzyme; Glomerular cell; Lipid peroxidation; |
語 文 | 英文(English) |
英文摘要 | We evaluated the effects of zinc deficiency on the activities of endogenous antioxidant enzymes and lipid peroxidation in rat glomerular cells (GCs). Male Sprague-Dawley rats (n=48) were fed a zinc-deficient diet and deionized distilled water for 1 week to induce zine deficiency. Half of the rats (zinc-deficient group) continued on this diet for 4 weeks, and the other half (zinc-replete group) were maintained on the same diet but with zinc-supplemented water(150mg/Lzinc sulfate solution). Half of each group underwent five-sixths nephrectomy, while the other half underwent a sham operation. Another 12 normal rats (controls) were fed standard rat chow(containing 23.4% protein and 70 ppm zinc) and drank deionized distilled water. The zinc-deficient rats, including sham and five-sixths nephrectomizied rats, showed severe growth retardation and poor appetite. Their mean plosma zine concentrations were half that of normal control rats, but their plasma copper concentration was significantly higher than that of the control rats. Zinc supplementation corrected the abnormality of plasma zinc and copper concentrations and the loss of body weight in Zinc- deficient rats. Zinc-deficient rats exhibited lower renal creatinine clearance and higher GC-malondialdehyde(GC-MDA) than zinc-replete rats. The remnant kidney of all five-sixths nephrectomized rats, including zinc-deficient clearance and increased GC-MDA concentations. Zinc concentrations in the renal and zinc- replete rats, cortex were decreased in zinc-deficient rats and the activities of GC-superoxide dismutase and GC-glutathione peroxidase were increased, while zinc -replete rats exhibited normal of Gc-superoxide dismutase and GC-glutathione peroxidase. We suggest that zinc deficiency enhances the formation of reactive oxygen species but does not affect the activities of endogenous antioxidant enzymes in glomerular cells. |
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