查詢結果分析
來源資料
相關文獻
- Comparisons of in Vitro Antioxidant Activities of Storage Proteins in Tuber of Two Dioscorea Species
- 山藥之品種特性、生產潛力、物化性質與抗氧化研究
- 山藥之生產潛力與抗氧化酵素活性之比較
- 山藥生粉之理化性質與抗氧化活性之研究
- 山藥生粉取代部分麵粉對山藥土司製造之影響
- 不同熱處理對紫玉山藥萃取液抗氧化性之探討
- 山藥抗氧化之研究(1):低密度脂蛋白過氧化系統之檢測
- Dioscorins from Different Dioscorea Species All Exhibit Both Carbonic Anhydrase and Trypsin Inhibitor Activities
- 山藥的保健效能與應用
- 山藥釀造酒之抗氧化研究
頁籤選單縮合
題名 | Comparisons of in Vitro Antioxidant Activities of Storage Proteins in Tuber of Two Dioscorea Species=兩品種山藥塊莖儲藏性蛋白質體外抗氧化活性之研究 |
---|---|
作者 | 劉玉華; 梁弘人; 鄭惠川; 劉燕雯; 侯文琪; | 書刊名 | Botanical Studies |
卷期 | 47:3 民95.07 |
頁次 | 頁231-237 |
分類號 | 341.94 |
關鍵詞 | 抗氧化; 儲藏性蛋白質; 胃蛋白酶水解物; 山藥; Antioxidant; Dioscorin; Peptic hydrolysates; Storage protein; Yam; |
語文 | 英文(English) |
中文摘要 | 兩品種山藥《台農一號與日本山藥》塊莖儲藏性蛋白質dioscorin經由DE-52離子交換層析法純化。經由一系列體外抗氧化實驗,包括清除DPPH與氫氧自由基,還原能力,抗脂質過氧化能力,保護去氧核糖核酸傷害能力,及抑制peroxynitrite氧化dihydrorhodamine 123能力。結果顯示,兩品種山藥dioscorin具有不同的抗氧化能力,即使100℃加熱五分鐘也仍具有不同的清除DPPH與氫氧自由基的能力。以胃蛋白酶水解台農一號dioscorin不同時間之水解產物也具有清除DPPH自由基的能力。以Sephadex G-50(F)膠濾層析進行分離並進行DPPH自由基清除實驗,結果顯示小分子水解產物也具有抗氧化能力。 |
英文摘要 | Dioscorin was purified by DE-52 ion exchange chromatography from two yam species, Dioscorea alata L. cv. Tainong 1 (TN1) and Dioscorea batatas Decne (imported from Japan, JP). By different in vitro antioxidant tests, including DPPH radical and hydroxyl radical scavenging activity assay, a reducing power test, an anti-lipid peroxidation test, DNA damage protection, and inhibition of dihydrorhodamine 123 oxidation by peroxynitrite, it was shown that dioscorins from the two species exhibited different scavenging activities against DPPH and hydroxyl radicals, even after heating 100℃ for 5min. Dioscorins from TN1 were hydrolyzed by pepsin for different durations and the peptic hydrolysates exhibited DPPH radical scavenging activities. Peptic hydrolysates separated by Sephadex G-50(F) gel filtration were tested for anti-DPPH radical activity. Results showed that fractions of smaller molecular weight still have antioxidant activities. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。