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題 名 | HPLC/Colorimetry Combination Method for Quantitative Analyses of Sclerotiorin Produced by Penicillium Sclerotiorum=以高效液相層析/比色分析組合法定量Penicillium Sclerotiorum生產之Sclerotiorin |
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作 者 | 翁順祥; 蘇南維; 鍾玉明; 李敏雄; | 書刊名 | 藥物食品分析 |
卷 期 | 12:3 2004.09[民93.09] |
頁 次 | 頁199-204+287 |
分類號 | 412.37 |
關鍵詞 | 高效液相層析; 比色分析法; 定量分析; Penicillium sclerotiorum; Sclerotiorin; HPLC/colorimetry combination method; Quantitative analyses; |
語 文 | 英文(English) |
中文摘要 | 本研究擬建立一種在無法獲得純標準品之情況下,利用HPLC配合比色法 (HPLC/colorimetry combination method) 定量Penicillium sclerotiorum菌體中sclerotiorin含量之方法。菌體以甲醇萃取後,採用C18逆相管柱進行HPLC分析 (流動相為乙腈水=65/35,v/v;檢測波長為370nm) 同時進行比色分析 (測定OD370) ,可以HPLC測得之面積百分比和比色法測得之OD370之關係所導出之方程式計算出菌體中sclerotiorin之含量。結果顯示,最低檢測濃度 (LOQ) 為0.5μg/mL,回收試驗之回收率為97-109%,變異係數均在3.8%以下,分析一個樣品,僅需約30min,相當簡便。 |
英文摘要 | In this study, quantitative analysis of sclerotiorin from Penicillium sclerotiorum was established by using HPLC/colorimetry combination method at the condition of unavailability of authentic sclerotiorin compound. Mycelium was extracted with methanol, then analyzed by HPLC (a C18 reverse phase column with acetonitrile/water = 65/35, v/v as mobile phase and detected at 370 nm) and simultaneously measured OD370 with spectrophotometers. The limit of quantitation (LOQ) was 0.5 mg/mL. Recovery studies were performed using the lyophilized mycelia, with each spiked with sclerotiorin at 1 and 10 mg, respectively. The recoveries were found in the range of 97~109%. The coefficients of variation were less than 3.8% with this HPLC / OD370 combination method. |
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