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題 名 | 川芎 於大白鼠腦部各區之濃度分佈研究=Regional Brain Distribution of Tetramethylpyrazine in Rat |
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作 者 | 梁健成; 洪傳岳; 陳介甫; 蔡東湖; | 書刊名 | 中醫藥雜誌 |
卷 期 | 10:2 1999.06[民88.06] |
頁 次 | 頁117-123 |
分類號 | 418.52 |
關鍵詞 | 川芎 ; 高效液相層析法; 定量; 腦組織; Tetramethylpyrazine; High-performance liquid chromatography; Quantification; Brain tissue; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究應用一套靈敏、可靠的高效液相層析法,來定量川芎�虳韝j白鼠腦組織各 區濃度。 以甲醇與水 (50:50,v/v,pH3.0 以正磷酸調整 ) 當作移動相,使用碳 18 的逆相 分離管柱 (4.6X250 mm,粒徑 5nm),流速為每分鐘 1ml 進行待測物分離,並以紫外光檢測 器 280 nm 為偵測波長。 腦組織最低可定量偵測極限為 0.1ug/g,每次取 100ug 來作分析 。 實驗以 10mg/kg 川芎�衁瑣窄q靜脈注射 15 分鐘後,迅速將大白鼠斷頭犧牲,並將其腦 組織分成六個部分 (大腦皮質、腦幹、紋狀體、海馬迴、小腦及中腦 ),分析川芎�虷b各區 之濃度、結果顯示,投藥後腦組織六個部分的川芎�衁瑪@度呈現均勻的分佈,且周邊血漿川 芎�衁瑪@度約高於腦組織五倍,靜脈注射後約 17% 的川芎�苭i以通過血腦障壁。 |
英文摘要 | We used a rapid and reliable high-performance liquid chromatographic method for determination of tetramethylpyrazine in rat brain. The separation was achieved on a Cosmosil 5C18 column (4.6 x 250 mm, particle size 5 um). The mobile phase consisted of methanoi-water (50:50, v/v, pH 3.0 adjusted by orthophosphoric acid) at a flow rate of 1.0 mL/min. Tetramethylpyrazine was monitored at a wave- length of 280 nm. The lower limit of quantification in brain tissue was O.1 ug/g, and only a small amount of brain tissue (100 ug) was required for analysis. Concentrations of tetramethylpyrazine in various regions of the brain (cerebral cortex, brainstem, striatum, hippocampus, cerebellum and mid- brain) were not significantly different at 15 min afteradministration (10 mg/kg, i.v.). Mean concentra- tion of the tetramethylpyrazine in plasma was approximately five times greater than in brain tissue. In additional analysis, about 17% of tetramethylpyrazine may penetrate blood-brain-barrier. |
本系統中英文摘要資訊取自各篇刊載內容。