查詢結果分析
相關文獻
- Determination of Methyl-and Inorganic Mercury in Fish Using Focused Microwave Digestion Followed by Cu亇Addition, Sodium Tetraethylborate Derivatization, η-Heptane Extraction, and Gas Chromatography-Mass Spectrometry
- 替代燃料對汽柴油引擎性能、毒性、污染與噪音特性分析之研究
- 蒸煮及鹽酒的添加對魚肉脂肪酸組成及脂質安定性之影響
- 固相微量萃取法(SPME)在酒類揮發成份分析上之應用
- 液化石油氣汽車引擎性能、毒性、污染與噪音特性分析及預測模式建立之研究
- 混合魚肉條乾品質之探討
- 以氣相層析質譜方法及氘同位素藥物為內部標準品應用於定量尿液中安非他命之探討
- Evaluation of Isotopic Analogs as Internal Standard for Quantitative Determination of Urinary 6-Acetylmorphine by Gas Chromatography/Mass Spectrometry
- 以多重細菌素之乳酸菌生產發酵鯖魚香腸之探討
- Preparative Isolation and Gas Chromatography-Mass Spectrometry Analysis of Triterpenoids in Kansui Radix
頁籤選單縮合
題名 | Determination of Methyl-and Inorganic Mercury in Fish Using Focused Microwave Digestion Followed by Cu亇Addition, Sodium Tetraethylborate Derivatization, η-Heptane Extraction, and Gas Chromatography-Mass Spectrometry=以聚焦式微波輔助萃取、乙基化及氣相層析質譜法分析魚肉中甲基汞及無機汞含量 |
---|---|
作者姓名(中文) | 陳石松; 周薰修; 黃登福; | 書刊名 | 藥物食品分析 |
卷期 | 12:2 2004.06[民93.06] |
頁次 | 頁175-182+197 |
分類號 | 412.37 |
關鍵詞 | 微波輔助萃取; 乙基化; 氣相層析質譜儀; 魚肉; 甲基汞及無機汞; Methylmercury; Inorganic mercury; Microwave-assisted digestion; Ethylation; GC-MS; Fish; |
語文 | 英文(English) |
中文摘要 | 本研究探討以微波輔助萃取、乙基化及氣相層析質譜法分析魚肉中甲基汞及無機汞含量。將檢體0.5g置微波消化瓶中,加四甲基氫氧化銨溶液5mL,以聚焦式微波消化器加熱萃取後,以醋酸中和,加入醋酸銅溶液及醋酸緩衝溶液 (pH5) ,再加入四乙基硼酸納/氫氧化鉀溶液衍生化,以正庚烷振盪萃取,靜置分層後取上層液以氣相層析質譜儀分析。實驗結果顯示,氣相層析質譜儀分析可於8分鐘內完成分析 (不含前處理時間) 。最佳微波萃取條件為15-30W加熱3.5分鐘、45W加熱2.5分鐘或60-75W加熱1.5分鐘。添加0.5,1.0及2.0μg/g甲基汞及無機汞於魚肉中,其回收率分別為92.3-96.1%及93.6-95.5%。其次,分析三種參考物質之甲基汞含量,BCR CRM464 tuna fish為5.31 ± 0.32μg/g (標示值5.50 ± 0.17μg/g) ,NRCC DORM-2dogfish muscle為4.40 ± 0.34μg/g (4.47 ± 0.32μg/g) 及DOLT-2 dogfish liver為0.663 ± 0.061μg/g (0.693 ± 0.053μg/g) ,顯示本方法適用於魚肉中甲基汞及無機汞含量之分析。 |
英文摘要 | The analytical procedure for analysis of methyl- and inorganic mercury in fish was developed in this study. It involved microwave-assisted digestion with alkaline solution (tetra methyl ammonium hydroxide), addition of Cu++, aqueous-phase derivatization of mercury species with sodium tetraethylborate, and subsequent extraction with n-heptane. The various mercury derivatives were desorbed in the splitless injection port of a gas chromatograph and subsequently analyzed by electron impact mass spectrometry. Optimum conditions allowed the sample throughout to be controlled by the instrumental analysis time (about 8 min per sample) but not by the sample preparation step. At the irradiation power of 15-30, 45, and 60-75 W, sample heating times were only 3.5, 2.5, and 1.5 min, respectively. The recoveries of mercury species were 92.3–96.1% and 93.6–95.5% for methyl- and inorganic mercury, respectively. The proposed method was finally validated by the analysis of three biological certified reference materials (BCR CRM 464 tuna fish, NRC DORM-2 dogfish muscle, and NRC DOLT-2 dogfish liver). |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。