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題 名 | A Rapid and Simple Gas Chromatographic Method for Direct Determination of Nicotinamide in Commercial Vitamins and Tonic Drinks=市售維生素飲料及口服液中菸鹼醯胺之直接注入氣相層析快速定量分析法 |
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作 者 | 林秀蓉; 陳重文; 黃寶雄; 鍾玉明; | 書刊名 | 藥物食品分析 |
卷 期 | 8:2 2000.06[民89.06] |
頁 次 | 頁113-123 |
分類號 | 412.37 |
關鍵詞 | 維生素飲料; 口服液; 菸鹼醯胺; 直接注入法; 氣相層析; 定量分析; Tonic drink; Nicotinamide; Direct injection; Gas chromatography; Quantitative analyses; |
語 文 | 英文(English) |
英文摘要 | A simple and rapid method was developed to determine the presence of nicotinamide in tonic drinks using the megapore semi-polar column (CP-Sil 8 CB, 30 m x 0.53 mm) with direct injection gas chromatography. Direct quantitative analysis of nicotinamide in amino acid drinks, vitamin drinks, essence of chicken and tonic drinks was carried out without any sample pretreatment procedure. Water soluble compound 1,9-nonanediol was used as an internal standard. The detection limit for nicotinamide was 2-5 μg/mL. Recovery studies were performed using 1 mL of vitamin drink and tonic drink, each spiked with nicotinamide at 105.5, and 211.0 μg, respectively. The recoveries were found in the range of 94~99% and 93-108%, respectively. The coefficients of variation being less than 9.8%. Forty-two commercial amino acid drinks, vitamin drinks, essences of chicken and tonic drinks were analyzed with this method. The nicotinamide contents were found as: 131-246, 24-112, 0-134 and 0-263 μg/mL, respectively. These results indicated that, one bottle each of the amino acid drink, vitamin drink, essences of chicken and tonic drink contain nicotinamide levels of : 39-74, 5-22, 0-10 and 0-21 mg/bottle. The results also showed that in 12 out of 4 of commercial amino acid and vitamin drinks, the nicotinamide exceeded the ROC's acceptable daily intake (RNDA, 14.4 mg) level; and 3 out of 10 commercial tonic drinks exceeded the ROC's RNDA level for nicotinamide. |
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