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頁籤選單縮合
題 名 | Polyunsaturated Fatty Acid Production with Mortierella alpina by Solid Substrate Fermentation=以Mortievella alpina利用固態發酵生產多元不飽和脂肪酸 |
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作 者 | 詹鴻得; 林裕益; 楊盛行; | 書刊名 | Botanical Bulletin of Academia Sinica |
卷 期 | 41:1 2000.01[民89.01] |
頁 次 | 頁41-48 |
分類號 | 434.241 |
關鍵詞 | 多元不飽和脂肪酸; 米糠; 固態發酵; Mortierella alpina; Polyunsaturated fatty acid; Rice bran; Solid substrate fermentation; |
語 文 | 英文(English) |
中文摘要 | 以Mortievella alpina利用固態發酵可以生產多元不飽和脂肪酸, 農業廢棄物中,米糠是最佳基質,而後依次為為花生粕、麥麩和甘藷渣。固態發酵生產多元不飽和脂肪酸之最適條件以米糠為基質,添加2.3至5.0%氮源在初水分含量65∼68%和初pH6∼7。培養8∼12天,每克基質碳源可得122.2 mg總多元不飽和脂肪酸,包括12.8 mg EPA,47.8 mg LA,7.1 mg ALA和54.5 mg ARA。基質C/N比介於14.5至18.5有利EPA和LA生產,而C/N比介於19.8至21則有利ARA和總多元不飽和脂肪酸、EPA和ARA產量分別提高12.2,84.4和46.1%。如於基質中額外添加1%大豆油或棉籽油則可分別提高 LA 產量 84.9 和36,ARA產量71和42.1%與EPA產量130.6和92.1%。 |
英文摘要 | Polyunsaturated fatty acids (PUFA) were produced with Mortierella alpina by solid substrate fermenation. Rice bran was the most effective substrate for PUFA production, followed by peanut meal residue, wheat bran, and sweet potato residue. The optimal conditions for PUFA production were rice bran supplemented with 2.3 to 5% nitrogen at an initial moisture content of 65 to 68% and a pH range of 6 to 7. Each gram of substrate carbon yielded 122.2 mg of total PUFA, including 12.8 mg of eicosapentaenoic acid (EPA), 47.8 mg of linoleic acid (LA), 7.1 mg of α-linolenic acid (ALA), and 54.5 mg of arachidonic acid (ARA) for 8 to 12 days incubation. C/N ratios between 14.5 and 18.5 favored EPA and LA production, while C/N ratios between 19.8 and 21 enhanced ARA and total PUFA production. Total PUFA, EPA and ARA production increased 12, 84.4 and 46.1%, respectively, when the culture temperature was shifted from 20 ℃ to 12 ℃ on the fifteh day. Supplement of soybean and linseed oils increased LA by 84.9 and 36%, ARA by 71 and 42.1%, and EPA by 130.6 and 92.1%, respectively. |
本系統中英文摘要資訊取自各篇刊載內容。