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頁籤選單縮合
題名 | 本土黑殭菌以固態和液態發酵生產黑殭菌素之探討=Production of Destruxins from a Local Entomopathogenic Fungus, Metarhizium anisopliae var. anisopliae, by Solid-State and Submerged Fermentation |
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作者姓名(中文) | 謝建元; 洪文凱; 高穗生; 王順成; 曾耀銘; | 書刊名 | 中國農業化學會誌 |
卷期 | 36:4 1998.08[民87.08] |
頁次 | 頁371-379 |
分類號 | 433.3 |
關鍵詞 | 黑殭菌; 黑殭菌素; 固態發酵; 液態發酵; Metarhizium anisopliae var. anisopliae; Destruxins; Solid-state fermentation; Submerged fermentation; |
語文 | 中文(Chinese) |
中文摘要 | 分離自紅胸葉蟲之本土黑殭菌小孢子變種,其所生產的黑殭菌素以質譜儀鑑定結 果, 五個主要菌素分子量分別為 577Da、564Da、579Da、594Da、594Da 證實為 DA、DA2、 DMDB、DB、及 DE。 以液態發酵培養和固態發酵培養 14 天後,無論以產孢生殖方式或菌絲 生殖方式皆能生產黑殭菌素。液態發酵培養,第七天達到最高菌體濃度,而黑殭菌素(以 DA、DB,和 DMDB 標的之總量)則在第 14 天時達到最高,但並不隨發酵間之增加。產率上 每公升培養液(含有 30g 的醣類為碳源), 所產得黑殭菌素含量依次為 1.57mgDB, 1.16mgDMDB,0.34mg DA 和 0.14mg DE,而 DA2 和 DD 則含量最少。 而固態培養中每克米 粒約可產 2 × 10 個孢子, 菌素的產率每 100g 乾重之米粒所產黑殭菌素含量依次為 4mgDA,3.03 mg DMDB, 2.29mg DB,0.95mg DE 和 0.21mg DA2,而DD含量較少。 |
英文摘要 | A local Entomopathogenic Fungus, Metarhizium onisopliae var. anisopliae , isolated from Brontispa longissima Gestro, was used for destruxin production. Both solid-state and submerged fermentation were able to produce destruxins after 14 days of fermentation. The major destruxins produced had molecular weights of 577 Da, 564 Da, 579 Da, 594 Da, and 594 Da, which corresponded to DA, DA2,DMDB,DB,and DE, respectively, as confirmed by their mass spectrum. With submerged cultural fermentation, the cell density reached up to 8 g/L after 7 days of fermentation, and the maximum destruxin concentration was schieved after 14 days of cultivation. The results showed when 30 g/L bactosaccharose was used in submerged fermentation, the destruxin yield was 1.57 mg DB, 1.16 mg DMDB, 0.34 mg DA, and 0.14 mg DE. With solid-state fermentation, it was found that 100 g of rice in dry weight yielded 4 mg DA, 3.03 mg DMDB, 2.29 mg DB, 0.95 mg DE, and 0.21 mg DA2 and produced 2 ×10 spore per gram of rice. |
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