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來源資料
頁籤選單縮合
題名 | 不同頻率及電針鎮痛對於脊髓5-羥色胺及類鴉片接受体之作用機轉研究=Studies of Mechanisms of Electroacupuncture Analgesia in Different Frequencies on Spinal Serotonergic and Opioid Receptors |
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作者姓名(中文) | 蔡輝彥; | 書刊名 | 中醫藥年報 |
卷期 | 17:2 1999.05[民88.05] |
頁次 | 頁201-243 |
分類號 | 413.91 |
關鍵詞 | 不同頻率電針; 鎮痛作用; 5-羥色胺接受器; 類鴉片接受器; Different frequencies; Analgesia; Serotonergic receptors; Opioid receptors; |
語文 | 中文(Chinese) |
中文摘要 | # 330 0$a類鴉片物質(opiate-like substance,OLS)及5-羥色鞍(5-HT, serotonin) 媒介針刺之鎮痛作用, 已見於發表之文獻, 且不同亞型之類鴉片接受器 (opioid receptor) 可能媒介不同頻率電針之鎮編作用;至於不同亞型之 5- 羥色胺接受器 (5-HT receptor) 在不同頻率電針鎮痛作用中扮演何種角色,尚未有進一步的報導。故本研 究在探討脊髓中,不同亞型之類鴉片接受器及 5- 羥色胺接受器在電針鎮痛之作用機轉。由 大鼠髓鞘內插管給藥並以足三里 (S36) 為針刺穴位。 在電針鎮痛預試實驗中,於大鼠甩尾 試驗結果顯示,低電流量 (1mA) 電針顯現較明顯鎮痛作用,而在較高電流量 (3mA) 電針於 重覆刺激後,其鎮痛作用減低。故本研究選用電流量 1mA,以不同頻率 (2Hz,10 Hz,100Hz) 電針刺激,探討不同接受器作用藥物對不同頻率電針鎮痛之影響。μ - 及δ - 類鴉片接受 器拮抗劑 naloxone 及 naltrindole 髓鞘內給藥均可拮抗低頻 (2Hz, 10Hz) 與高頻 (100Hz) 之電針鎮痛作用;但前者對於低頻的敏感性,大於高頻; 而後者對於高頻之敏感性 大於低頻。 5 羥色胺神經毒素 5,7-dihydroxytryptamine (5,7-DHT) 可以阻斷不同頻率電 針之鎮痛作用。 5-HT1A 接受器拮抗劑 pindobind-5-HT1A 0.5、5 μ g 可減低不同頻率之 電針鎮痛, 5-HT1A 接受器致效劑 8 OH-DPAT0.5 μ g 可拮抗高頻電針之鎮痛作用, 但 1 μ g 及 2 μ g 可加強低頻電針鎮痛作用。 5 . HT2/1C 接受器拮抗劑 ketanserin 0.5 μ g 減弱低頻電針鎮痛, 5-HT2/1C 接受器致效劑 DOI 10 μ g 並未影響電針鎮痛作用。 5-HT3 接受器拮抗劑 LY-278584 0.5、5 μ g 可明顯減弱高頻電針鎮痛,5 μ g 則對低頻 之電針鎮痛亦有減弱作用; 5-HT3 接受器致效劑 2-methy1-5-HT 50 μ g 則可加強電針鎮 痛作用。由以上結果顯示,在脊髓中μ - 及δ - 類鴉片接受器均參與電針之鎮痛作用,而 μ - 類鴉片接受器對低頻電針作用較敏感;而δ - 類鴉片接受器則對高頻電針作用較敏感 。 5- 羥色按接受器 5-HT1A、 5-HT2/1C 及 5-HT3 顯現均與電針之鎮痛作用有關,至於主 要是影響脊髓中突觸前或突觸後的 5-HT1A、5-HT2/1C 及 5-HT3 接受器, 則有待進一步之 探討。 |
英文摘要 | In this study, the effect of antinociception in different current of lectroacupunctue(EAc) was studied; the electroacupuncture analgesia (EAA) effect of I mA EAc is better than 2 mA, 3 mA EAc; the EAA could keep more stable and longer; thus I mA EAc was chosen for the consequence study. Naloxone could block the EAc analgesia in three frequencies (2 Hz, 10 Hz, 100 Hz); that suggested (receptor might participate the low and high frequencies of EAc analgesia. Besides, naltrindole ((receptor antagonist) blocked the high frequency of EAc analgesia, suggesting (receptor might participate the high frequency of EAc analgesia. 8-OH-DPAT (0.5 (g, i.t.) might elevate the pain threshold in rat, but the antinociceptive effect of I (g, 2 (g were lower than the effect of 0.5 (g 8-OH-DPAT. 5-HTIA receptor might play a role of inhibition of antinociception pathway. 8-OH-DPAT (1 (g) might potentiate the pain threshold on low frequency of EAc; no significant effect under 1 (g of 8-OH-DPAT. It suggest that 5-HTIA was involved in the low frequency of EAA. the 5-HT2/IC antagonist, ketanserm, could attenuate the lower frequency of EAc(2 Hz and 10 Hz); but not affect the 100 Hz of EAc. It suggested that 5-HT2/IC receptor might be involved in low frequency of EAc. 5-HT2/IC agonist, DOI, could not affect the EAc in different frequencies. (-, (-opioid receptor and 5-HTIA and 5-HT2/IC, 5-HT3 may be involved in electroacupuncture analgesia in different mechanisms. |
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