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| 題 名 | 中樞單胺及類鴉片亞型接受體在不同頻率電針引起鎮痛機轉之研究=Studies of Action Mechanisms in Different Requencies of EAC Analgesia on Central Monoaminergic and Opioid Receptors |
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| 作 者 | 蔡輝彥; | 書刊名 | 中醫藥年報 |
| 卷 期 | 16:1 1998.05[民87.05] |
| 頁 次 | 頁233-260 |
| 分類號 | 414.5 |
| 關鍵詞 | 不同頻率電針; 鎮痛機轉; 中樞單胺及類鴉片亞型接受體; Different frequent electroacupuncture; Analgesic effect; Central monoaminergic and opioid subtype receptors; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 針刺鎮痛及針刺麻醉的機轉,近幾十年來有許多學者持續地進行研 究,而隨著疼痛機轉的不斷瞭解,針刺鎮痛的機轉亦被證實與中樞神經 系統中的神經enkephalin、endorphin及單胺類物質5-HT、NE的濃度 有關。本研究利用福馬林試驗來探討三種不同頻率(2 Hz、10 Hz、100 Hz)電針鎮痛之機轉與單胺神經原及類鴉片接受體之間的關係;更進一 步利用高效液相色層分析儀(HPLC)來測定三種不同頻率電針對於腦內單 胺物質的影響。 結果顯示:(1)5-HT 10 ug/kg、30 ug/kg(i.c.v.)可加強高頻 100 Hz 電針之對於中樞鎮痛作用;而30 ug/kg可加強不同頻率之周邊 鎮痛作用。NE 3、10、30 ug/kg(i.c.v.)皆可加強高頻(100 Hz)之鎮 痛作用。(2)PCPA (i.p.)可逆轉三種頻率電針之鎮痛效果;5-HTP (i.p.)則加強三種頻率電針之鎮痛效果;而α-MT可加強高頻率(100 Hz)電針對中樞之鎮痛作用及三種頻率電針對周邊之鎮痛作用。(3) Naloxone可逆轉2 Hz和10 Hz電針在中樞之鎮痛效應,但無法逆轉 高頻(100 Hz)之作用;而naltrindole 則逆轉 10 Hz和100 Hz電針 在中樞之鎮痛效應,但無法逆轉低頻(2 Hz)之作用。(4)Prazosin (i.p.)及clonidine (i.p.)皆可加強三種頻率電針之鎮痛作用,然而 yohimbine (i.p.)則會逆轉2 Hz 及10 Hz之電針鎮痛作用,但卻加強 100 Hz之電針鎮痛作用。(5)Pindobind-5-HT1A及 LY-278584 皆會逆 轉三種頻率電針鎮痛作用,而ketanserin 則加強了100 Hz電針之 鎮痛作用。由以上結果顯示,三種頻率之電針鎮痛機轉,與單胺類物質 NE及5-HT皆有關,在電針後5-HT主要可能經由5-HT1A及5-HT3接 受體來媒介,而高頻率電針可能還有5-HT2接受體的參與;在腎上腺素 系統中則α1及α2接受體皆有參與,且可能扮演著相反的角色。另 外,在低頻率(2 Hz)電針刺激下與u-類鴉片接受體的關係較密切, 而高頻率(100 Hz)的刺激則與δ-類鴉片接受體較有關。 |
| 英文摘要 | There are many studies on the mechanism of antinociception of electroacupuncture (EAc) in the past decades. It has been proved that antinociception of EAc was related to the concentration of neuropeptides (enkephalin, endorphin) and monoamines (5HT, NE). In order to understand the relationships of different frequencies of EAc analgesia on monoaminergic neurons and opioid receptors, this study was accomplished by the formalin test in ICR mice. The concentrations of endogenous monoamines were determined by HPLC. The evidences suggest that (1)Exogenous 5-HT and NE enhanced the analgesic effect of the different frequencies of EAc, especially at 100 Hz. (2)The antinociception of EAc at different frequencies stimulation (2, 10 and 100 Hz) were attenuated by PCPA, and were potentiated by 5-HTP. (3)Naloxone could reverse the EAc analgesia of 2Hz and 10Hz but not 100 Hz. However, natrindole could reverse the EAc analgesia of 10 Hz and 100 Hz but not 2 Hz. (4)Prazosin and clonidine could potentiate the antinociception of different frequencies of EAc whereas yohimbine could reverse 2 Hz and 10 Hz EAc analgesia and potentiate 100 Hz EAc analgesia. (5) Pindobind-5-HT1A and LY-278584 could reverse the three different frequencies of EAc analgesia and ketanserine potentiate 100 Hz EAc analgesia. The concentractions of brain endogenous monoamines were influenced by different frequencies of electroacupuncture. From the above results, we suggest that the analgesic effect of EAc is related to serotonergic and noradrenergic neurons at different frequency stimulation. In serotonergic pathway, EAc analgesia may be mediated via 5-HT1A and 5-HT3 receptors. Besides, 5-HT2 may be involved in high frequency EAc analgesia. In noradrenergic pathway, both α1 and α2 receptors were involved in EAc analgesia and may be play in the opposite function. Moreover, the EAc analgesia may be mediated via u- opioid receptor at low frequency stimulation and δ-opioid receptor at high frequency stimulation. |
本系統中英文摘要資訊取自各篇刊載內容。