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題 名 | GAs Chromatography-Mass Spectrometry Analysis of 11-Nor-9-Carboxy-△勄-Tetrahydrocannabinol in Urine=以氣相層析質譜儀分析尿中大麻代謝物 |
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作 者 | 林棟樑; 郭宗禮; 劉瑞厚; | 書刊名 | 藥物食品分析 |
卷 期 | 7:1 1999.03[民88.03] |
頁 次 | 頁37-43 |
分類號 | 418.21 |
關鍵詞 | 氣相層析質譜分析法; 氘內標準品; 尿中大麻代謝物; 定量分析; 11-nor-9-carboxy-△勄-tetrahydrocannabinol; GC-MS; Internal standard; Urine; Drug of abuse; |
語 文 | 英文(English) |
中文摘要 | 本研究係以氣相層析質譜分析法來定量尿中大麻代謝物11-nor-9-carboxy-△�e -tetrahydrocannabinol(THC-COOH)。 經評估 d �� - 與 d �� -THC-COOH 二種內標準品後 , 發現 d �� -THC-COOH 對尿中大麻代謝物並無任何干擾定量分析之離子,適合 GC-MS 做 定量分析。 本實驗採三甲基矽化 (trimethylsilyl) 衍生物, 以離子監測法 (SIM, Selected Ion Monitoring) 來定量, 監測離子 THC-COOH 為 m/z 371,473,488, 內標準 d �� -THC-COOH 為 m/z 380,479,497。 以 2ml 空白尿液配製濃度在 10 至 200 ng/ml 範圍 內, 其抽取回收率平均約為 90 %, 日間與日內精密度試驗,其變異係數分別為 0.35 ∼ 5.31 %與 0.44 ∼ 5.19 %,並具有良好的線性關係 r �� >0.999。 |
英文摘要 | Effective specimen pretreatment and GC-MS protocols were developed for the analysis of 11-nor-9-carboxy- △�e -tetrahydrocannabinol (THC-COOH)in urine. d �� -and d ��-11-nor-9-car- boxy- △�e -tetrahydrocannabinol (d �� -and d �� -THC-COOH) were evaluated as internal standards and conclusions were reached that THC-COOH can be effectively analyzed, qualitatively and quantitatively, as trimethylsilyl derivative using selective ion monitoring of the following ions: m/z 371, 473 and 488 (for THC-COOH) and m/z 380, 479 and 497 (for d �� -THC-COOH). The extraction procedure achieved 90% recovery of the analyte in fortified drug-free urine containing 10-200 ng/ml THC-COOH. Using 2-ml sample size, the overall protocol resulted in the following analytical parameters in the 10-200 ng/ml concentration range studied:interday and intraday precision ranges, 0.35-5.31% and 0.44-5.19%, respectively; linearity, r �� >0.999. |
本系統中英文摘要資訊取自各篇刊載內容。