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題名 | 花生未熟胚培養的研究(4):未熟胚軸及子葉不同部位培養體胚形成之探討=Studies on the Immature Embryo of Peanut (Arachis hypogaea L.) Ⅳ: In Vitro Embryogenesis from Immature Embryo Axes and Cotyledons |
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作者 | 鄭皓鴻; 葉茂生; | 書刊名 | 農林學報 |
卷期 | 47:1 1998.03[民87.03] |
頁次 | 頁91-105 |
分類號 | 434.257 |
關鍵詞 | 花生; 未熟胚培養; 體胚形成; Peanut; Immature embryo culture; Embryogenesis; |
語文 | 中文(Chinese) |
中文摘要 | 利用栽培種花生(Arachis hypogaea)台南選9號(TNS9)及台南11號(TN11)之未熟胚 軸及子葉之三種不同部位為培植體,分別為(1)切除胚根及胚芽之胚軸(代號A),(2)切除頂 端之上半部子葉(代號B-),(3)切除基部之下半部子葉(代號C-),以L□□為基礎培養基, 添加2mg/l NAA + 1mg/l BA之A2培養基為對照,將添加之NAA、BA濃度改變為2:0.5,2:2, 1:1,3:1,代號為A7∼A10等5種培養基,做為體胚形成之誘導培養基,進行花生未熟胚軸 及子葉不同部位之體胚形之培養,結果如下:未熟胚軸的體胚、芽體形成率及平均體胚數均 高於未熟子葉,且均為直接體胚形成。五種培養基之體胚誘導率呈極顯著差異,誘導之體胚 多為正常之體胚。以A2培養基誘導之體胚、芽體形成率及平均誘導的體胚數最高。癒合組 織的誘導率,以A9培養基最高;根的形成率則以A8培養基的誘導率最高。 |
英文摘要 | This study attempted to compare the in vitro embryogenesis on different explants of peanut on different media components. Immature embryo axes and cotyledons from immature seeds of peanut cultivars TNS9 and TN11 were classified into three explant types, including embryo axis without radicle and epicotyl (A), the distal half cotyledon without tip(B-), and the proximal half cotyledon without base (C-). Five media, denoted as A2, A7, A8, A9 and A10, were used for embryogenesis. The A2 medium was the L□ basal medium supplemented by 2 mg/l NAA+1 mg/l BA. The other media, i.e., A7~A10, were changing the concentration of NAA and BA in the A2 medium into 2 mg/l NAA+0.5 mg/l, 2 mg/l NAA+2 mg/l BA,l mg/l NAA+l mg/l BA and 3 mg/l NAA+l mg/l BA, respectively. Results are summarized as follows: The rates of embryogenesis and shoot formation and the mean number of embryos produced from immature embryo axes were higher than those from immature cotyledons. Among the five media tested, the A2 medium was the highest in embryogenesis and shoot formation, and the greatest in the mean number of the embryos. The rates of embryo formation in the five embryogenes media varied significantly. Most embryos initiated were normal. Different types of callus were produced in different media. The highest rate of callus formation was found in A9 medium. The A8 medium was better for root formation. |
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