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題名 | 花生未熟胚培養的研究(2):不同胚齡及種子大小之未熟胚培養對癒合組織及體胚形成的影響 |
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作者 | 葉茂生; 賴媛敏; | 書刊名 | 農林學報 |
卷期 | 44:1 1995.03[民84.03] |
頁次 | 頁67-85 |
分類號 | 434.253 |
關鍵詞 | 花生; 未熟胚培養; 體胚形成; 胚齡; 種子大小; |
語文 | 中文(Chinese) |
中文摘要 | 本研究以栽培種花生台南選9號(TNS9)及台南11號(TN11)為材料,取受精 後子房柄伸入土表後9~24天不同胚齡之未熟胚為培植體,以L2為基礎培養基,添 加2mg/1NAA+1mg/1BA(代號A2),以MSE為基礎培養基,添加2.5mg/l2,4-D(A4), 以及MS為基礎培養基,添加1mg/12,4-D(A5);1mg/12,4-D+1mg/1NAA(A6)等四種培 養基,探討比較不同胚齡及種子大小乏未熟胚培養時對癒合組織及體胚形成的影 響,結果如下: 就胚齡而言,11天以下的未熟胚其體胚形成率普遍低落,只在培植體表面有切刻 的部位形成白色或淺綠色疏鬆的癒合組織,無進一步分化;12~16天左右的未熟 胚,在伸展開的子葉或原生的胚芽旁長出1~數個體胚,17天至20天左右的胚軸之 體胚形成率亦相當高。 種子長度3mm以下的未熟種子不適於胚培養,體胚形成率幾乎為零,4~6mm左右 者取出整個未熟胚培養,則可得較高的體胚形成率,而6mm以上,則因培養基的 不同而有所差異,除A2培養基外,其餘三種培養基對較長之未熟種子形成體胚 的誘導效果不大。 就四種培養基而言,A2培養基的體胚形率較高,產生多量且正常的體胚,並進 而發育成芽體:A4~A6培養基均能誘導出多量的癒合組織,及少量的體胚。最適 合花生未熟胚培養的胚齡是在子房柄伸入地下後14-20天,而種子長度約5~8mm。 |
英文摘要 | The object of this study is to investigate the effect of embryo ages and seed length oncallus formation and somatic embryogenesis from immature embryos of peanut. Two cultivars,Tainan Selected 9 (TNS9) and Tainan 11 (TN11), and four media were used in the experiment. An A2 medium using L2 medium as the basis, were supplemented with 2 mg/1 NAAand 1 mg/1 BA. An A4 medium was prepered by adding 25 mg/1 2,4-D into the MSB basalmedium. The mediium A5 and A6 were prepered by using MS medium as the basis andadding 1 mg/1 2,4-D and 1 mg/1 2,4-D + 1 mg/1 NAA, respectively. The results are summarized as follows: Immature embryos with an age under 11 days showed low somatic embryogenesis frequency. They produced white or light green calli which no any differentiation were found.However, one or several somatic embryos were observed beside the cotyledons or the originalembryo axes derived from 12 ~ 16-day immature embryos. And the 17 ~ 20-day immature axesgot rather high somatic embryogenesis frequency. The immature seed shorter than 3 mm scarcely gave somatic embryogenesis frequency.However, the intact immature embryos cut from seeds with 4~6 mm in length had highfrequency of embryogenesis. As to the 6 mm or longer seeds, their somatic embryogenesLs ratedepended on the media. Of the four media used in this study, A2 medium that induced a large amount ofnormal somatic embryos was a suitable medium for embryogenesis. The other media. A4, A5and A6, induced a great amount of callus but a few somatic embryo. For peanut embryog.enesis, immature embryos must develope in 14 ~ 20 days after gynophore penetrating into thesoil or get from seeds with 5 ~ 8 mm in length. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。