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題名 | 飼料中添加幾丁質對斑節蝦成長及免疫力之影響=Effects of Diets Containing Chitin on Growth and Immunocompetence of the Kuruma Shrimp Penaeus Japonicus |
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作者 | 張錦宜; 李武忠; 陳淑鈴; 徐崇仁; Chang, Chin-i; Lee, Wu-chung; Chen, Shu-ling; Shyu, Chung-zen; |
期刊 | 水產研究 |
出版日期 | 19970600 |
卷期 | 5:1 1997.06[民86.06] |
頁次 | 頁11-19 |
分類號 | 439.66 |
語文 | chi |
關鍵詞 | 斑節蝦; 幾丁質; 酚氧化酵素; Kuruma shrimp; Chitin; Phenoloxidase; |
中文摘要 | 本研究以兩次實驗的結果來評估飼料添加幾丁質對斑節蝦Penaeus japonicus成長及免疫力的影響。實驗一:於飼料中添加0、200、400、600、800及1000 mg╱kg幾丁質分組餵飼四十天後,發現餵食400、600、800及1000 mg╱kg幾丁質添加飼料的斑節蝦成長率顯著(P<0.05)優於餵食0及200 mg╱kg幾丁質添加飼料者。同樣餵食四十天的斑節蝦以肌肉注射5×10�� CFU╱g BW Vibrio vulnificus PJ1進行感染試驗,連續觀察二週,記錄死亡情形。餵食400、200及600 mg╱kg幾丁質添加飼料的斑節蝦活存率顯著(P<0.05)高於餵食0、800及1000 mg╱kg幾丁質添加飼料者。餵食400、600及800 mg╱kg幾丁質添加飼料的斑節蝦血淋巴酚氧化酵素(Phenoloxidase,PO)活性亦高於餵食200及1000 mg╱kg者,而餵食0 mg╱kg者其血淋巴PO活性最低。實驗二:以400 mg╱kg幾丁質添加飼料連續餵食不同天數後再改餵未添加幾丁質的飼料,以探討飼料中添加幾丁質的最適餵養天數之結果顯示,需連續餵食10天才有提高斑節蝦抵抗力的效果,而效期至少可持續5天。 |
英文摘要 | Two series of experiments were conducted to evaluate the effectiveness of dietary chitin on the growth and immunocompetence of the kuruma shrimp Penaeus japonicus. In the first experiment kuruma shrimp were fed with diets containing graded levels of chitin (0, 200, 400, 600, 800 and 1000 mg/kg diet) for 40 days. The growth rate observed in the 400, 600, 800, 1000 mg/kg groups were significantly (p<0.05) higher than those 0 and 200 mg/kg groups. When challenged by intramuscular injection of Vibrio vulnificus PJ1 at a dosage of 5 × 10�� CFU/g body weight, the shrimp fed with 400, 200 and 600 mg/kg chitin showed survival rates significantly (p<0.05) higher than the shrimp fed with 0, 800 and 1000 mg/kg chitin. Additionally, the shrimp fed with chitin at concentrations of 400, 600 and 800 mg/kg showed an enhanced phenoloxidase (PO) activity than the 200 and 1,000 mg/kg groups, and the group fed with 0 mg/kg chitin showed the lowest PO activity. In the second experiment shrimps were fed with diet containing 400 mg/kg chitin for various time periods to investigate the optimum duration to maximize the protection. The results revealed that the protective effect was highest after 10-day continuous administration and lasted for at least five more days. |
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