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頁籤選單縮合
題 名 | Chitinase Activity of Sweet Potato (Ipomoea batatas [L.] Lam var. Tainong 57)=甘薯中的幾丁質酶 |
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作 者 | 侯文琪; 陳盈州; 林耀輝; | 書刊名 | Botanical Bulletin of Academia Sinica |
卷 期 | 39:2 1998.04[民87.04] |
頁 次 | 頁93-97 |
分類號 | 434.313 |
關鍵詞 | 幾丁質酶; 甘薯; 防禦角色; Chitinase; Defense against pests; Ipomoea batatas; |
語 文 | 英文(English) |
中文摘要 | 以商品乙二醇幾丁質為基質,我們檢測到甘薯不同部位酵素粗抽液中的幾丁質�t 的活性。若以每克新鮮重量中的單位活性表示,則粗抽液中的幾丁質�t活性的大小次序為: 葉子>芽>塊根的皮>去皮的塊根。在田間種植的甘薯中,葉子的幾丁質�t活性高於蔓。被 蟲咬過的葉子顯然比對照組(完整的葉),有比較高的幾丁質�t的活性。連接於被蟲咬過的 葉子的蔓比連接於對照組的蔓,有較高的幾丁質�t的活性。依據活性染色,甘薯葉子中的主 要幾丁質�t的分子量為 16 kDa,最適反應 pH 為 5.0,最適反應溫度為 25 ℃。 |
英文摘要 | Chitinase activity was detected in the crude extracts of different parts of sweet potato (Ipomoea batatas [L.] Lam var. Tainong 57) using commercial deacetyl glycol chitin as a substrate. The activity of chitinase in crude extracts, which was expressed as units/g fresh weight, was arranged in decending order of leaves > sprouts > peels of storage roots > peeled storage roots. Under field conditions, the chitinase activity of leaves was higher than vines. The chitinase activity of insect-bitten leaves was higher than that of the controls, and the chitinase activity of vines attached to insect-bitten leaves was also higher than that of vines attached to intact leaves. The molecular weight of main chitinase determined by activity staining was about 16 kDa. The optimum pH was 5.0 and the optimum temperature of chitinase activity was 25 ℃. |
本系統中英文摘要資訊取自各篇刊載內容。