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題名 | Purification and Characterization of a 94 KD High Molecular Weight Allergen from House Dust Mite, Dermatophagoides Pteronyssinus=94KD高分子量家塵蟍過敏原之純化與特性分析 |
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作者 | 魏俊傑; 李學孚; 張東玄; 周正成; 謝貴雄; 黃智雄; | 書刊名 | 中華民國微生物及免疫學雜誌 |
卷期 | 30:4 1997.11[民86.11] |
頁次 | 頁228-241 |
分類號 | 414.8 |
關鍵詞 | 家塵蟍; 過敏原; House dust mite; Dermatophagoides pteronyssinus; Allergen; IgE; mAb; |
語文 | 英文(English) |
中文摘要 | 在許多國家,家塵�虩堥t中Dermatophagoides pteronyssinus (Dp)是引起過敏性氣喘及鼻炎的主要過敏原。其中大部分小分子量的過敏原已被確認,而高分子量過敏原的研究卻十分有限。本論文研究一萃取自家塵�薽芶斢妗悛囿�94KD高分子量過敏原,並進行純化與特性分析。我們利用家塵�薽芶斢妗悛咩K疫BALB/c小白鼠產生特異性抗體後,再進行融合瘤生產,得到2205-3.54與2220-7.25兩株單株抗體,認知家塵�藃颩鴗�94KD之蛋白質成分。利用單株抗體製備高親合性管柱與HPLC配合,純化出此--94KD蛋白質並分析其過敏原特性。針對四十位已知對Dp過敏的氣喘病童之血清學試驗,ELIDA結果發現有15位病童血清含認知該94KD蛋白質的IgE抗體、佔37.5%。再對20名氣喘病童進行皮膚試驗,顯示9位病童有正反應(佔45%),明顯高於正常人。實驗結果証明此--94KD家塵�藅捰角嶼偌飪M存在於台灣的重要過敏原。 |
英文摘要 | House dust mite allergens from Dermatophagoides pteronyssinus is an important cause of severe allergic asthma and rhinitis in many countries. Although several low to medium molecular weight allergens had been well characterized, limited studies on the high molecular weight IgE-binding components were reported. In this study, a 94 kD high molecular weight allergen from crude mite body extract of D. pteronyssinus was purified and characterized. Monoclonal antibody (mAb) affinity chromatography and high performance liquid chromatography were used to purify 94 kD allergen. Its antigenicity and allergenicity were confirmed by in vitro and in vivo studies. Two mAbs 2205-3.45 and 2220-7.25 specific to 94 kD high molecular weight component of D. pteronyssinus were generated. The epitopes recognized by these mAbs were species-specific. Enzyme-linked immunosorbent assay (ELISA) of IgE reactivity in the sera from 40 asthmatic children allergic to D. pteronyssinus showed that 37.5% of them had significantly higher optical density values (range 0.011 to 0.452) than normal (range 0.013 to 0.035). In in vivo skin test showed that 9 out of 20 (45%) asthmatic children were positive to 94 kD allergen. The results demonstrate that 94 kD high molecular weight component is an important allergen existing in house dust mite in Taiwan. |
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