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題名 | Aspergillus Niger生產葡萄糖氧化酵素之研究=A Study of Glucose Oxidase Production by Aspergillus Niger |
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作者 | 李泰興; | 書刊名 | 技術學刊 |
卷期 | 12:4 1997.12[民86.12] |
頁次 | 頁635-640 |
分類號 | 463.35 |
關鍵詞 | 葡萄糖氧化酵素; 醱酵; Glucose oxidase; Fermention; Aspergillus niger; |
語文 | 中文(Chinese) |
中文摘要 | Aspergillus niger是一株好氧性之菌株,在醱酵 24 小時後溶氧幾乎為零,氧氣 成為生長之限制基質。而溶氧之限制對菌體生產葡萄糖氧化酵素之影響,比菌體生長較為敏 感。Aspergillus niger 產生的葡萄糖氧化酵素可分為胞內與胞外,而胞內約占 82 %。而 菌體之形態與溶氧有關,在對數生長期由小菌球,慢慢變成大菌球,而因溶氧不足後,才慢 慢產生菌絲,最後大部以菌絲形態存在。在溶氧為 6.2ppm 以下則會影響到菌體生產葡萄糖 氧化酵素的能力(菌體之比活性)。而就菌體生長、基質消耗及葡萄糖氧化酵素生產的關係 而言,Aspergillus niger 是屬增殖連動型( growth-associated type )。 |
英文摘要 | In this study Aspergillus niger was classified as an aerobic fungus. Dissolved oxygen was almost zero after 24 hours of the fermentation process and oxygen became the limiting substrate of growth. Dissolved oxygen was more sensitive in limiting the productin of glucose oxidase than limiting cell growth. Glucose oxidase produced by Aspergillus niger was classified as an intracellular and extracellular enzyme and intracellular glucose oxidase was about 82%. The morphology of the colony was closely related to the dissolved oxygen. A small pellet which was inoculated into the fermenter slowly grew to a large pellet during the exponential growth phase. Then the pellet was partially transformed into filamentous mycelium under the insufficiently dissolved oxygen condition and finally almost all the pellet became filamentous mycelia. The ability of the cell to produce glucose oxidase was decreased when dissolved oxygen was below 6.2 ppm. As for fungus growth, substrate consumption and glucose oxidase production, Aspergillus niger was categorized as a growth-associated type. |
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