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題 名 | Correlation of Interleukin-1β, Interleukin-6, and Periodontitis=介白質1β、介白質6與牙周炎之關係 |
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作 者 | 陳慶長; 張基隆; 黃正芬; 黃景勝; 蔡吉政; |
書刊名 | The Kaohsiung Journal of Medical Sciences |
卷 期 | 13:10 1997.10[民86.10] |
頁 次 | 頁609-617 |
分類號 | 416.946 |
關鍵詞 | 牙周炎; 介白質-1β; 介白質-6; Interleukin-1β; Interleukin-6,; Periodontitis; |
語 文 | 英文(English) |
中文摘要 | 本研究之目的,第一部分:在比較牙周炎患者與牙周健康者之牙齦組織與血清中介白質1β (IL-1β) 和介白質6 (IL-6) 之濃度,並探討牙齦組織中此兩種介質濃度與牙周臨床參數之關係,以便實際瞭解IL-1β與IL-6在發炎組織中所占的角色。第二部分:以體外實驗,探討IL-1β刺激健康牙齦造纖維細胞產生IL-6之程度,藉以瞭解二者之互動關係。第一部分實驗取材包括:(1) 成年型牙周炎病患組,33個病變組織與8個健康組織標本,以及10個病患之血清標本;(2) 牙周健康對照組,15個正常組織和10個健康者之血清標本。所獲得之組織與血液經處理與離心後,以酵素免疫分析法測定細胞激素濃度。結果顯示:(1) IL-1β與IL-6在牙周炎病患組之病變組織中,有顯著增加,(2) IL-1β與IL-6和牙周臨床參數 (囊袋探測深度、附連高度、齒槽骨吸收高度) 呈有意義之正相關,(3) 在牙周炎病患組之組織中,IL-1β與IL-6濃度呈有意義之正相關 (r=0.4334,P<0.01),(4) 牙周炎病患組與牙周健康對照組之間,血清中IL-1β與IL-6濃度沒有統計上之差異。第二部分實驗用造纖維細胞,分別培養自6位牙周健康者之牙齦組織。實驗時,細胞培養於24孔培養皿中,達融合時加入濃度分別為0.05、0.5、5、50、500單位/毫升之重組人類介白質1β (rhlL-1β),作用48小時後,取上層培養液經離心、過濾,然後利用促進IL-6-dependent hybridoma cell line 7TD1增殖能力之生物分析法來測量IL-6活性。結果顯示:(1) 6株造纖維細胞分泌IL-6濃度,在添加rhlL-1β後均比未添加rhlL-1β時(對照組)呈有意義增高,且均隨rhlL-1β濃度增加而增加,(2) 各細胞株之間,對rhlL-1β感受性互有差異,其中3株細胞顯示具有較高分泌IL-6之能力。因此,由本研究可得以下結論:牙齦組織中IL-1β與IL-6濃度多寡和牙周炎之嚴重程度有密切關連,但牙周炎患者血清中IL-1β與IL-6濃度並沒有增加的現象;另外,IL-1β刺激牙齦造纖維細胞產生IL-6之程度,除了與IL-1β之濃度有關係外,也會因細胞來源個體之不同而有所差異。因此,可以推測,在牙周疾病的過程中,發炎細胞產生之IL-1β可能會刺激牙齦造纖維細胞產生大量IL-6,進而有助於牙周組織之破壞。 |
英文摘要 | In order to understand the role of IL-1β and IL-6 in the periodontal tissue destruction coincident to periodontitis, we assessed the levels of these two mediators in both the gingival tissue and the serum of patients with periodontal disease and of periodontally healthy subjects. In addition, production of IL-6 by six healthy human gingival fibroblast (HGF) strains in response to IL-1β was also investigated. The levels of IL-1β and IL-6 in gingival tissues and in serum were examined by ELISA. Both mediators were observed to increase in diseased tissues of patients with adult periodontitis, and there was a positively significant relationship between both mediators and clinical assessments of periodontal destruction. Moreover, a significant correlation was also noted between levels of IL-1β and IL-6 in gingival tissues of periodontitis patients (r=0.4334, p<0.01). However, there was no significant difference in the serum levels of IL-1β and IL-6 between periodontitis patients and periodontally healthy controls. In fibroblast cultures, confluent monolayers of HGF were incubated with recombinant human IL-1β for 48 hat 37℃ in 5% CO2 and air. At the end of the culture period, supernatants were collected and assayed for IL-6 activity by inducing proliferation in the IL-6-dependent hybridoma cell line 7TD1. A dose-dependent stimulatory effect of IL-1β on IL-6 production by HGF was noted, wherein 3 strains exhibited higher IL-6 activity than the other 3. These data indicate that the levels of IL-1β and IL-6 In gingival tissues are closely related to the severity of periodontal disease and that the IL-1β and IL-6 produced in gingival tissues may not reflect these two mediators levels in serum. Moreover, IL-1β responsiveness of HGF in IL-6 production depends on both the concentration of IL-i 0 and cells of individual subjects. Since HGF are present in periodontal lesion, it is possible that IL-6 secretion stimulated by exposure to inflammatory cell products such as IL-1β may participate in the destruction of periodontal tissue in periodontitis. |