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題 名 | Interleukin-10 Administration Inhibits TNF-α and IL-1β but Not IL-6, Secretion of LPS-Stimulated Peritoneal Macrophages=酯多醣刺激之腹腔巨噬細胞外加介白質-10可抑制其腫瘤壞死因子-α及介白質-1β而非介白質-6之分泌 |
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作 者 | 林金源; 唐菁吟; | 書刊名 | 藥物食品分析 |
卷 期 | 15:1 2007.03[民96.03] |
頁 次 | 頁48-54+95 |
分類號 | 418.2216 |
關鍵詞 | 介白質-10; 介白質-1β; 腫瘤壞死因子α; 介白質-6; 脂多醣刺激的腹腔巨噬細胞; Interleukin-10; IL-10; IL-1β; IL-6; Tumor necrosis factor-α; TNF-α; Lipopolysaccharide-stimulated peritoneal macrophages; LPS-stimulated peritoneal macrophages; |
語 文 | 英文(English) |
中文摘要 | 本研究旨在探討抗發炎細胞激素介白質-10,額外添加至脂多醣刺激的腹腔巨噬細胞培養基中,對其促發炎細胞激素介白質-1β、介白質-6及腫瘤壞死因子(tumor necrosis factor,TNF)-α分泌之影響。實驗進行首先自BALB/c雌鼠之腹腔取得初代腹腔巨噬細胞,並以脂多醣(lipopolysaccharide,LPS)刺激以活化巨噬細胞,在48 hr培養期間內的不同時間點,測試培養液中抗發炎及促發炎細胞激素分泌量的變化。結果發現,隨培養時間增加,LPS刺激所產生之介白質-1β、6、10及腫瘤壞死因子-α的濃度亦隨之增加,其分泌濃度分別為介白質-6 > 腫瘤壞死因子-α >介白質-10 > 介白質-1β。在培養18 hr後,內生性介白質-10含量下降,為探討外加介白質-10之抗發炎作用,因此選擇在脂多醣刺激巨噬細胞後18 hr,外加不同濃度(0、75、150及225 pg/mL)抗發炎細胞激素介白質-10 至細胞培養液中,並持續培養至48 hr,以觀察外加介白質-10 對已活化巨噬細胞分泌細胞激素之影響,結果發現,外加介白質-10 抑制介白質-1β(21.3~38.6%)及腫瘤壞死因子-α(44.7~66.8%)之分泌量,且以添加低劑量(75 pg/mL)介白質-10有最大效果,但對介白質-6之分泌則無顯著影響。另外發現,外加介白質-10,可增加培養液中總介白質-10之濃度(18.4%~35.5%),但只有外加低劑量介白質-10可增加內生性介白質-10之分泌量10.4%,外加較高劑量之介白質-10,反而抑制內生性介白質-10之分泌量達31.7至41.9%。綜合本實驗結果推測,添加低劑量介白質-10可經由抑制腫瘤壞死因子-α及介白質-1β,而非介白質-6 之分泌,同時刺激內生性介白質-10之分泌,而達到抗發炎之功效。 |
英文摘要 | We hypothesize that exogenous administration of anti-inflammatory cytokine interleukin (IL)-10 would affect the secretion of pro-inflammatory cytokines such as IL-1β, IL-6, or tumor necrosis factor (TNF)–α using lipopolysaccharide (LPS)-stimulated macrophages exuded from the peritoneal cavity of female BALB/c mice. The LPS-stimulated macrophages were incubated for 48 hr to investigate the secretions of pro- and anti-inflammatory cytokine. The results indicated that the secretion levels of IL-1β, IL-6, IL-10, and TNF–α by LPS-stimulated macrophages elevated in a time dependent manner during 48-hr incubation period. The amount of cytokines secretion by LPS-stimulated macrophages varied: IL-6 > TNF-α > IL-10 > IL-1β. However, the secretion of IL-10 started to decrease at 18 hr of incubation. Therefore, the macrophage cultures were extra-administrated with various concentrations (0, 75, 150, and 225 pg/mL) of exogenous IL-10 after 18-hr incubation in order to evaluate the effect of exogenous IL-10 administration on inflammation. IL-10, IL-1β, IL-6, and TNF–α in LPS-stimulated macrophage cultures before and after exogenous IL-10 administration were determined by ELISA. The results demonstrated that exogenous IL-10 administration inhibited IL-1β (21.3-38.6%) and TNF–α (44.7-66.8%) secretion. However, the administration did not inhibit IL-6 secretion. Low dose (75 pg/mL) of exogenous IL-10 exerted maximal effects. IL-10 levels in LPS-stimulated macrophage cultures after exogenous IL-10 administration increased from 18.4% to 35.5%. Furthermore, only low dose of exogenous IL-10 administration increased endogenous IL-10 secretion (by 10.4%), while higher doses of exogenous IL-10 inhibited endogenous IL-10 production (from 31.7% to 41.9%). The results suggest that low dose administration of exogenous IL-10 might exhibit anti-inflammatory effects via inhibiting TNF-α and IL-1β, but not IL-6, secretion and increasing endogenous IL-10 production by LPS-stimulated peritoneal macrophages. |
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