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題 名 | Human Hepatic Alcohol and Aldehyde Dehydrogenases: Genetic Polymorphism and Activities=人肝醇及醛脫氫酶:遺傳多型性與活性 |
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作 者 | 姚鍾太; 廖錦霞; 尹士俊; | 書刊名 | Proceedings of the National Science Council : Part B, Life Science |
卷 期 | 21:3 1997.07[民86.07] |
頁 次 | 頁106-111 |
分類號 | 415.121 |
關鍵詞 | 人肝醇; 醛脫氫酶; 遺傳多型性; 活性; Genetic polymorphism; Liver; Alcohol dehydrogenase; Aldehyde dehydrogenase; Activity; |
語 文 | 英文(English) |
中文摘要 | 醇脫氫�t( ADH )和醛脫氫�t( ALDH )是人體主要的酒精代謝 。此二�t在不 同種族表現遺傳多型性。我們研究 23 例肝手術檢體,探討酒精代謝�t活性與遺傳多型性間 的關係。 應用瓊膠等電焦集電泳鑑定 ADH 與 ALDH 同功�t表現型及紫外光可見光光譜儀動 力測定�t之活性。 在 pH7.5,33mM 乙醇反應條件,同型接合子表現型 ADH �� 1-1 和 ADH �� 2-2 及異型接合子表現型 ADH �� 1-2 肝檢體�t活性分別測得 2.9 ± 0.7, 16.0 ± 2.5 及 13.6 ± 1.0 單位╱克組織。在 200 μ M 乙醛濃度,ALDH2 活性型和 ALDH2 失活 型肝檢體�t活性分別測得 1.06 ± 0.13 及 0.71 ± 0.07 單位╱克組織。這項結果發現人 類肝臟的酒精代謝活性依 ADH �祟M ALDH �秣穧]位置多型性而顯著差異, 並顯示東方人族 群不同基因型肝之酒精代謝能力不同,會影響其飲酒行為與酒癮的發生。 |
英文摘要 | Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) are the major enzymes responsible for the metabolism of ethanol in the body. Both exhibit genetic polymorphism in racidal populations. To determine hepatic ethanol metabolizing activities in relation to genetic polymorphism, a total of 23 surgical specimens were investigated. The experession patterns of ADH and ALDH isoenzymes were identified by means of agarose isoelectric focusing, and the activities were assayed spectrophotometrically. At 33 mM ethanol, pH 7.5, the activities in the liver with the homozygous phenotype ADH �� 1-1 and ADH �� 2-2 and the heterzygous phenotype ADH �� 1-2 were determined to be 2.9 ± 0.7, 16.0 ± 2.5, and 13.6 ± 1.0U/G tissue, respectively. The activities of the ALDH2-active and ALDH2-inactive phenotypes at 200 μ M acetal-dehyde were determined to be 1.06 ± 0.13 and 0.71 ± 0.07 U/g tissue, respectively. These findings indicate that human hepatic ethanol-metabolizing activities differ significantly with respect to polymorphism at both the ADH �� and ALDH �� loci. The results suggest that this genetically determined differential hepatic activity may influence drinking behavior and the development of alcoholism among Orientals. |
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