相關文獻
- Cloning of Mercury Resistance Determinants in Escherichia coli and Analysis of Mercury Reduction Activity in vivo and in vitro
- 即食性沙拉用生菜中Escherichia coli O157:H7之污染
- 宜蘭縣主次要河川水體水質微生物含量變化之研究
- 不同淨水製程單元對市售衛生冰塊水質之影響
- 串聯電極式壓電石英晶體感測器之多通道系統於大腸桿菌的總生菌數檢測
- 大腸桿菌群檢驗方法介紹
- Over-expression and Characterization of Copper/Zinc-superoxide Dismutase from Rice in Escherichia Coli
- 如何檢測與防治漢堡中毒菌--大腸桿菌O157:H7血清型
- 錐形瓶中氣液質傳係數及好氣培養菌體濃度之估算方法
- Protease-Like Sequence in Hepatitis B Virus Core Antigen is Not Involved in the Cleavage Processes of Core Protein in Escherichia coli
題 名 | The Seventh Copy of IS1 in Escherichia Coli W3110 Belongs to the Is1A (IS1E) Type Which is the Only IS1 Type That Transposes from Chromosome to Plasmids=大腸桿菌W3110品系中第七套IS1屬於IS1A(IS1E)種類並且此種類是W3110中唯一會自染色體轉位到質體的一個IS1種類 |
---|---|
作 者 | 陳建華; 葉修足; |
書刊名 | Proceedings of the National Science Council : Part B, Life Science |
卷 期 | 21:3 1997.07[民86.07] |
頁 次 | 頁100-105 |
分類號 | 414.83 |
關鍵詞 | 大腸桿菌; IS1; PCR; Transposition; |
語 文 | 英文(English) |
中文摘要 | 大腸桿菌 K-12 品系染色體上已知有六套 IS1 ( IS1A-IS1F )。 依據其核甘酸 序列, 可分成四種:IS1A ( IS1E ), IS1B ( IS1C ), IS1D, IS1F。 但 Zuber 與 Schumann 於 1993 年進一步於 W3110 品系的 49.6 分鐘位置又發現有第七套 IS1 的存在 ,並且發表此套 IS1 尾端 21-bp 及其外圍約 120-bp 的序列。我們根據 IS1A ( IS1E ) , IS1B ( IS1C ),IS1D 的前端 16 鹼基序列及第七套 IS1 尾端外圍鹼基的序列合成二 個引子,將 W3110 染色體上第七套 IS1 以聚合�t連鎖反應的方法複製出來,並加以定序。 結果顯示第七套 IS1 序列與 IS1A ( IS1E )序列完全相同。 我們進一步利用一質體系統 篩選染色體 IS 轉位到質體的質體自發性突變株,總共得到 142 株質體 IS 轉位株。 這其 中有 38 株為 IS5 之轉位株,10 株為 IS30 之轉位株, 而其餘 94 株為 IS1 之轉位株。 以限制脢圖譜分析此 94 株 IS1 轉位株, 我們發現其皆為具有 IS1A ( IS1E )序列的 IS1 轉位株。 |
英文摘要 | In the Escherichia coli K-12 chromosome, six copies of IS1 (IS1A-IS1F) have been identified and characterized. According to their nucleotide (nt) sequences, the six IS1 copies can be classified into four types, IS1A (IS1E), IS1B (IS1C), IS1D and IS1F type. Zuber and Schumann (1993) identified the seventh IS1 copy at 49.6 minutes on the E. coli W3110 genetic map. Unfortunately, only the end 21-bp sequence as well as the neighboring 120-bp E. coli sequence were reported. We therefore disigned two oligonucleotide primers to specifically amplify the seventh IS1 copy by PCR. One primer is homologous to the first sixteen bases of the IRL sequence of IS1A (IS1E), IS1B (IS1C) and IS1D. The other primer is complementary to the eighteen bases of E. coli sequence adjacent to IRR of the seventh IS1 copy. An 800-bp PCR fragment was obtained and its nt sequence determined, revealing an identical nucleotide sequence to that of IS1A (IS1E). A plasmid system was then used to isolate insertion mutations caused by insertions of the chromosomal insertion sequences. Of the 142 plasmid insertion mutants isolated, thirty-eight were insertions of chromosomal IS5. ten were IS30, and ninety-four were IS1. Detailed restriction mapping indicates that all ninety-four plasmid IS1 insertions were insertions of IS1 of the IS1A (IS1E) type. |