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頁籤選單縮合
題名 | 濾泡液對豬卵母細胞於離體(in vitro)成熟作用之影響=Effects of Supplement of Porcine Follicular Fluid on Porcine Oocytes Maturation in Vitro |
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作者 | 鄭三寶; 黃尉東; 朱志成; 吳信志; | 書刊名 | 中國畜牧學會會誌 |
卷期 | 26:1 1997.03[民86.03] |
頁次 | 頁37-53 |
分類號 | 437.21 |
關鍵詞 | 豬卵母細胞; 於離體成熟率; 胚分裂率; 濾泡液; 血清; Porcine oocyte; Maturation in vitro; Cleavage rate; Follicular fluid; Serum; |
語文 | 中文(Chinese) |
中文摘要 | 本試驗之目的,在於探討於含激性腺素培養液中添加 0 (對照組)、 20 、40、 60、80 和 100% 等六種不等比例之豬卵巢濾泡液( porcine follicular fluid; pFF )或 母豬發情首日血清( Day 0 porcine serum; PS )對豬卵母細胞于體外之成熟作用, 以及 復經受精處理後胚之分裂率( cleavage rate )之影響。 當取自豬卵巢濾泡(直徑 3 ∼ 7 mm )之卵丘卵母細胞複合體( cumulus-oocyte complexes ), 逢機被置於含 20% 或以上( 40 ∼ 100% ) pFF 之基礎成熟培養液 [TCM - 199 + FSH ( 2.5 u g/ml ) + LH ( 5 IU/ ml ) ] 培養 44 ∼ 48 hr 時(處理一),卵母細 胞之成熟率( % of Met II stage )均顯著高於置於未添加(對照)組者( 49.3 ∼ 55.7% vs. 10.7%, P< 0.05 )惟添加 pFF 組別彼此間之差異均不顯著。卵母細胞經歷成熟 培養和受精處理 [ 經與體外獲能處理後之精子混合培養 8 ∼ 16 hr 並移入含 20% PS 之 mBMOC-2 繼續培養 72 ∼ 96 hr] 後,胚之分裂率較對照組者為佳( 36.9 和 37.1% vs. 12. 4%, P<0.05)。於評估卵母細胞置於含 20% pFF 或 PS (Day 0)培養液中之成熟作用之 資料(處理二)顯示,當培養時間增長時,卵母細胞之成熟率亦告增加。各組卵母細胞之成 熟率達峰值所需之培養時間,於基礎成熟培養液(對照組)為 44 hr (峰值為 42.6%),P S 組為 40 hr (72.5%),pFF 組為 36 hr (78.3%)。 資料提示,激性腺素( FSH、LH )與濾泡液( 20% 或以上)同時存在培養液中,將有助 於豬卵母細胞於體外成熟率,以及受精處理後胚之分裂率。 於培養液中添加 20% 之母豬血 清( day 0 )或濾泡液,均具有促進卵母細胞之成熟作用; 而濾泡液之參予,較之血清者 為甚。 |
英文摘要 | The objectives of this study were to evaluate the effects of supplemen t of porcine follicular fluid (pFF) or sow serum [collected at the first day of estrus (Day 0, PS)] in the medium containing gonadotrophins on porcine oocyte maturation and their subsequent development (cleavage rate) after fertilization in vitro. In trial I, the cumulus-oocyte complexes collected from the ovarian follicles (3 ∼ 7 mm in diameter) were randomly allocated to and cultured in the basal maturation media [TCM-199+FSH(2.5 u g/ml)+ LH(5 IU/ml)] containing 0 (control group), 20, 40, 60, 80 and 100% of follicular fluid, respectively, for 44 ∼ 48 hr. The maturation rate (% of Met II stage ) was significant higher in the pFF addition groups (20% or above )than that of in the basal medium only (49.3% ∼ 55.7% vs. 10.8%, P<0.05). No significant differences among the pFF addition groups were found. After in vitro maturation, fertilization (co-cultured with capacitated sperm for 8 ∼ 16 hr) and cultivation in the mBMOC-2 medium supplemented with 20% porcine serum for 72 ∼ 96 hr, the differences in the cleavage rate among the pFF addition groups were not significant either. However, the cleavage rate in both of the 40 and 100% pFF addition groups were significantly higher than that of the control group (36.9% and 37.1% vs. 12.4%, P<0.05). Results indicated that the combination of gonadotrophins (FSH and LH) and follicular fluid in culture medium were beneficial to the in vitro maturation rate and subsequent cleavage rate after fertilization. The time required for/and the peak maturation rate of porcine oocytes in basal medium (control group) and in basal medium supplemented with 20% PS (Day 0) or pFF in trialII were evaluated during cultivation. They were 44 hr (42.6%), 40 hr (72.5%), and 36 hr (78.3%), respectively. Obviously, addition of 20% Day 0 porcine serum or follicular fluid to the medium containing gonadotrophins did exert influences on the maturation rate. Besides, the addition of follicular fluid might play a much more crucial role than the serum during the in vitro maturation process of porcine oocytes. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。