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題 名 | 利用雞毛分解菌行生物分解作用生產胺基酸之最適條件探討=Studies on the Optimum Conditions for Production of Amino Acids by Biodegradation with Feather-Degrading Bacteria |
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作 者 | 周淑芬; 高銘木; | 書刊名 | 嘉南學報 |
卷 期 | 22 1996.11[民85.11] |
頁 次 | 頁95-104 |
分類號 | 343.8 |
關鍵詞 | 雞毛分解菌; 生物分解效率; 胺基酸; 生物反應槽; Feather-degrading bacteria; Biodegradation efficiency; Amino acid; Bioreactor; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究係利用本實驗室先前自土壤篩選出之雞毛分解菌:AS-B4,BS-B1,CS-B2及CS-B4四株菌,進一步對其分解雞毛產生胺基酸之最適操作條件作一個探討。所探討之操作條件包括:溫度、pH、菌種儲存穩定性、雞毛濃度及培養天數等項目,並依所得最適條件之組合來建立一個簡易生物反應槽,期能提高雞毛分解效率及胺基酸之產量。由最適溫度試驗結果得知:AS-B4,CS-B2及CS-B4均以37°C時之雞毛分解效率最高,而BS-B1則以25~37°C最高;並發現雖同樣在最適溫度下,CS-B2、BS-B1及CS-B4之雞毛分解效率均明顯較AS-B4為高。於initial pH試驗中,發現BS-B1,CS-B2及CS-B4均以pH8.5左右最適;其中CS-B2之pH適應性最廣(pH7.1-8.5)。而菌種穩定性試驗結果則顯示CS-B2之貯存穩定性最高。綜 合以上結果,後續試驗選用於常溫操作之BS-B1及雞毛分牽效率和穩定性均較高之CS-B2,以利經濟需求。於雞毛濃度及培養天數試驗中顯示:BS-B1及CS-B2以2.5%之雞毛濃度培養九天較合乎經濟效益。最後設計並進行簡易生物反應槽之操作,結果發現:CS-B2之雞毛分解效率可提高至78.7%;BS-B1則為76.0%,頗具開發潛力,值得進一步研究。 |
英文摘要 | The purpose of this research is to set up the optimum conditions for production of amino acids by biodegradation with four previous selected feather-degrading bacteria (AS-B4, BS-B1, CS-B2 and CS-B4). The operating conditions included temperature, pH, storage stability, concentration of feather and incubation time. In the temperature test, the results showed that the optimum temperatures of AS-B4, CS-B2 and CS-B4 were 37°C; BS-B1 was 25-37°C. Because biodegradation efficiency of AS-B4 was the lowest, we deleted AS-B4 in the later tests. In initial pH test, the results indicated that the optimum pHs of BS-B1, CS-B2 and CS-B4 were pH8.5. In storage stability of feather-degrading bacteria test, the results showed that the stability of CS-B2 was the highest. Thus, both BS-B1 and CS-B2 were selected for the later tests to achieve the economical requirement. The concentration of feather and incubation time test showed that 2.5% of feather powder and 9 days of incubation time was the most economical conditions. Finally, the biodegradation efficiency of CS-B2 and BS-B1 was up to 78.7% 76.0% respectively, with a simple bioreactor. |
本系統中英文摘要資訊取自各篇刊載內容。