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題名 | 蝴蝶蘭病毒之特性及其防治 |
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作者 | 李惠鈴; | 書刊名 | 臺東區農業改良場研究彙報 |
卷期 | 6 1995.06[民84.06] |
頁次 | 頁1-23 |
分類號 | 435.432 |
關鍵詞 | 蝴蝶蘭; 齒舌蘭輪紋病毒; 喜姆比蘭嵌紋病毒; 工具消毒; Phalaenopsis spp; ORSV; CyMV; Tool treatment; |
語文 | 中文(Chinese) |
中文摘要 | 以雙層抗體直接(DAS-ELISA)法調查本省臺中及臺東兩地區七個蘭園中蝴蝶蘭植 株上發生CyMV(Cymbidium mosaicvirus)及ORSV(Odontoglossum rinespotvirus)的情形 CyMV及ORSV的感染率分別為41.24%及8.9%。將感染CyMY的蝴蝶蘭葉片小塊浸於0.l M 磷酸緩 衝液(PB)(PH7.2)中以微波爐50%火力加熱三十秒,即有固定CyMY帶狀內含體的效果, 0.l M PB中含0.25% Glutaraldehyde,則可增加固定的效果。蝴蝶蘭在接種CyMY 或ORSV 2O 個月後,以DAS-ELISA法偵測,在根、葉、花均有病毒存在;以免疫電顯捕捉法偵測,在感 染ORSV的花梗芽中,所有頂芽生長點均有病毒存在,側芽生長點則有87.5% 帶病毒;感染 CyMV者,頂芽生長點20%,而側芽生長點有30.77%病毒存在。將10個不同品種的蝴蝶蘭分別 接種ORSV、TMV(tobacco mosaic virus)、ToMV(tomato mosaic virus)及CGMMV (cucumber green mottle mosaic virus)等病毒,結果l0個品種均可被ORSV感染,而 TMV無法感染每一品種的全部接種植株,TohlV及CGMMV智不造成感染。以5%NaOH作為蘭園工 具消毒的藥劑,可防止ORSV、CyMV及ORSV與CyMV複石感染之傳播。 |
英文摘要 | Three hundred and twenty-four Phalaenopsis spp. samples collected from 7 orchid gardens in Taichung and Taitung district were surveyed for cymbidium mosaic virus (CyMV) and odontoglossum ringspot virus (ORSV) by using DAS-ELISA techniques. The incidence of CyMV and ORSV was 41.24% and 8.9%, respectively. CyMV banded inclusion can be fixed by microwave irradiation with 50% power for 30 sec. Glutaraldehyde can improve the fixation when microwave irradiation applies. CyMV and ORSV were distributed in the roots, leaves and flowers of inoculated Phalaenopsis detected by ELISA. In the immunospecific electron microscopy test, CyMV was present in approximately 20% of the apical buds and 30.77% of the lateral buds; ORSV was in every apical buds and 87.5% of the lateral buds. All the 10 Phalaenopsis cultivars were susceptible to ORSV, but not to tomato mosaic virus (ToMV) and cucumber green mottle mosaic virus (CGMMV). TMV only causes partial infection in each cultivar. In the chemical control test, CyMV or ORSV did not spread when cutting tools were treated with 5% NaOH. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。