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頁籤選單縮合
題 名 | One Step Filtration Method for Isolation of Herpesviral DNA=過濾方法分離皰疹病毒DNA |
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作 者 | 王孟亮; 張天傑; 王之仰; | 書刊名 | 臺灣畜牧獸醫學會會報 |
卷 期 | 65:2 1995.06[民84.06] |
頁 次 | 頁189-191 |
分類號 | 437.246 |
關鍵詞 | 去氧核糖核酸; 皰疹病毒; DNA isolation; Herpesvirus; |
語 文 | 英文(English) |
中文摘要 | 本報告提供一種快速又便宜的方法以分離病毒DNA。此方法具有三個優點:(1)操作時間大量減少;(2)減低細胞的用量,節省許多人力及物力;(3)適用的對象可涵蓋多種不同大小的病毒。 |
英文摘要 | A rapid method for the isolation of herpesviral DNA from cell culture is described. Relative to other previous reports, our method has three advantages: (1)The poeration time of other methods ranges from several hours to overnight; however, the key step in our method takes only about 20 minutes for the separation of viral DNA from most cellular DNA which is highly viscous. (2)The number of infected cells needed can be scaled down from 109 to 106; therefore, a tremendous labor of cell culture can be saved. (3)The method presented here can be applied to a broad spectrum of DNA virus such as SV40 (b~5 kbp), adenovirus(~36 kbp), and herpesvirus (>150 kbp). |
本系統中英文摘要資訊取自各篇刊載內容。