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頁籤選單縮合
題名 | Enzymatic Transglycosylation of Lactose in Reversed Micellar System=逆微胞中酵素催化糖基轉移反應 |
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作者 | 陳志成; 歐陽昭志; 葉智偉; 李宗翰; | 書刊名 | 大同學報 |
卷期 | 24 1994.11[民83.11] |
頁次 | 頁149-155 |
分類號 | 361.1925 |
關鍵詞 | 逆微胞; 酵素; 催化糖基; 轉移反應; |
語文 | 英文(English) |
中文摘要 | 於微水系統中,β-半乳糖水解酵素(大腸桿菌來源)被包覆於逆微胞中而進行乳 糖之糖基轉移反應,當水分含量控制在 Wo = 20 (水和介面活性劑之莫耳數比),乳糖被 轉化成異乳糖和三半乳糖寡糖(約 30% W/W ), 以及多糖基寡糖( d.p. ≧ 4. 約 15% W/W )。而於類似反應條件之水溶液系統中,只有極少數寡糖得以合成(< 4% w/w )。實 驗中亦發現 Wo 之下降有助於半乳糖寡糖之合成。 |
英文摘要 | b-galactosidase (E. coli) had been successfully enveloped into reversed micellar system with the micro-aqueous phase for the transglycosylation of lactose. Lactose had been transformed into allolactose, galactotrisaccharides (∼ 30% w/w) and higher galactooligosaccharides of degree of polymerization ≧ 4 (∼ 15% w/w) in reversed micelles with Wo = 20 (molar ratio of water to surfactant); under similar reaction conditions in aqueous reaction system, only trace amount of galactooligosaccharides (< 4% w/w) can be produced. It is also found that decreasing Wo will enhance the formation of galactooligosaccharides under the same degree of hydrolysis of lactose. |
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