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頁籤選單縮合
題 名 | 水稻縞葉枯病毒之純化及部份性質研究 |
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作 者 | 陳慶忠; 黃婉玲; | 書刊名 | 臺中區農業改良場研究彙報 |
卷 期 | 41 1993.12[民82.12] |
頁 次 | 頁33-42 |
分類號 | 434.111 |
關鍵詞 | 水稻縞葉枯病毒; 純化; 電子顯微鏡; 核蛋白; 血清類緣關係; Rice stripe virus; Purification; Electron microscopy; Nucleoprotein; Serological relationship; |
語 文 | 中文(Chinese) |
中文摘要 | 利用高低速交互離心及蔗糖梯度離心純化之縞葉枯病毒(rice stripe virus, RSV),A260/280比值為1.409±0.13,平均每公克水稻病葉可純化27.8病毒。純 化材料之準備以4月至6月接種發病者純化病毒濃度較高。純化病毒經SDS裂 解後以12.5%膠體電泳分析得到分子量34.7K鞘蛋白一種。前述純化病毒再經一 次硫酸銫等密度離心所得病毒材料以2%醋酸雙氧鈾溶液染色,可觀察到寬度約 10nm,長度不一,最長為40呈線形或環形之螺旋體構造的病毒顆粒。利用western blotting方法以RSV抗血清與RSV及玉米條紋病毒(maize stripe virus, MStV)抗原 作用時有免疫反應產生,但與水稻萎凋矮化病毒(rice wilted stunt virus, RWSV)則 無。ELISA以RSV抗血清與RSV抗原有專一性反應,但 與MStV及RWSV均 呈微弱免疫反應。 |
英文摘要 | Rice stripe virus (RSV) isolated in central Taiwan has been purified and partial characterized. RSV was purified from infected leaves of Oryza sativa L. by one cycle of differential centrifugation, followed by isopycnic centrifugation in cesium sulfate gradient. Preparation thus obtained has a maximum absorbance at 261 nm and a minimum at 236 nm. The A 260/280 ratio of purified nucleoprotein was 1.409±0.13. The yield of purified RSV was 7.2 to 53 with an average of 27.8 per gram of rice leaf tissue. Purified RSV stained with uranyl acetate, appeared as a helical structure about 10 nm wide. The thread-like filaments about 100-600 nm either appeared as irregular circular form or flexible filaments. Sometimes, uncoiled filaments or filaments with branches were also observed. One species of capsid protein subunit with molecular weights estimated as 34.7 K was obtained in the purified preparation of RSV by SDS- PAGE procedure. In western blotting test, the antiserum produced against RSV reacted specifically with RSV but not with two other tenuiviruses, rice wilted stunt virus (RWSV) and maize stripe virus (MStV). However, positive heterologous reactions were obtained between RSV antiserum and antigens of RWSV and MStV in indirect ELISA test, although the readings of the heterologous reation were relatively weak. |
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