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題 名 | 黃鰭鯛生長激素CDNA在大腸桿菌的表現 |
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作 者 | 林谷霖; | 書刊名 | 海軍軍官學校學報 |
卷 期 | 3 1993.12[民82.12] |
頁 次 | 頁165-172 |
分類號 | 387.101 |
關鍵詞 | 大腸桿菌; 生長激素; 黃鰭鯛; |
語 文 | 中文(Chinese) |
中文摘要 | 利用聚合�t鏈反應(polymerase chain reaction)將黃鰭鯛(Acanthopagrus latus HOUTTUYN,yp)生長激素(GH)cDNA合成為mature ypGH cDNA,再轉接到以噬菌體T7 Φ10為起動子的表現載體pET-3a上;所構築好的表現載體pETYP於大腸桿菌BL21(DE3)中表 現出來的生長激素約佔大腸桿菌總蛋白質含量的25.3%,其內函體蛋白質生長激素抗體的免 疫偵測為正反應。 |
英文摘要 | By combining the yellowfin porgy (Acanthopagrus latus HOUTTUYN, yp) growth hormone (GH) cDNA with Escherichia coli, the cDNA fragment of the mature ypGH was amplified by the polymerase chain reaction. The product was then ligated into the expression vector pET-3a, which is controlled by phage T7 Φ 10 as a promoter. As a result, the amount of ypGH produced by E. coli with the constructed vector i.e.,pETYP,was 25.3% of the total bacterial protein of E. coli BL21(DE3). Also, around 22 kilodaltons (kDa) of isolated inclusion body of the polypeptide immunorective to GH antibody. |
本系統中英文摘要資訊取自各篇刊載內容。